Abstract

Acarbose is widely used in medicine, such as the treatment of diabetes and obesity. A simple Thin Layer Chromatography (TLC)-scanning technique was developed for the rapid and accurate analysis of acarbose in a large number of fermentation broths of actinomycetes to screen for acarbose producer. The linearity of the acarbose in this way was good within the range from 2 to 10 μg (r² = 0.9997). This technique didn't need expensive instrument and complex procedure for the detection of acarbose in the fermentation broths. An actinomycete with acarbose yield of 1.83 mg/mL was obtained. The results demonstrated that TLC-scanning was a cheap and simple technique for the accurate screening of acarbose-producing actinomycetes.

Keywords:

Acarbose, actinomycetes, diabetes, mellitus, screening, TLC,

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