Abstract

Pigmented rice, consisting of black rice and red rice, is known to contain antioxidant compounds in its bran that tend to leach out into the water during cooking. As the rice is usually cooked in excess water which is discarded after cooking, the purpose of this study is therefore, to evaluate the rice cooking water as an antioxidant drink in terms of its antioxidant properties, storage stability and anthocyanin degradation kinetics. The results showed that the percentages of antioxidant extractability from pigmented rice into the cooking water were 88.42 and 103.26%, respectively for red rice and black rice, respectively. However, red rice drink possessed significantly (p<0.05) higher antioxidant activity than black rice drink, except for its total monomeric anthocyanin content. The drinks showed good microbiological stability throughout 12 weeks of storage when kept at 4°C, while those stored at 25°C lasted for 4 weeks. There was a significant decrease of antioxidant content, chroma and pH and increase in L value and hue angle, while less significant changes were observed for total soluble solids and viscosity of the drinks during the storage stability study. The degradation of anthocyanins in both drinks kept at different temperatures followed first-order reaction kinetics. According to the findings of this study, black rice and red rice cooking water have the potential of being new antioxidant drinks.

Keywords:

Anthocyanin, antioxidant drink, antioxidant properties, black rice, degradation kinetics, red rice, storage stability,

References

1. Abdel-Aal, E.M. and P. Hucl, 1999. A rapid method for quantifying total anthocyanins in blue aleurone and purple pericarp wheats. Cereal Chem., 76(3): 350-354.
   CrossRef    
2. Abdel-Aal, E.M., J.C. Young and I. Rabalski, 2006. Anthocyanin composition in black, blue, pink, purple and red cereal grains. J. Agr. Food Chem., 54: 4696-4704.
   CrossRef    PMid:16787017    
3. Ayo, J.A., 2004. Effect of acha (Digitaria exilis staph) and millet (Pennisetum typhodium) grain on kunun zaki. Brit. Food J., 106(7): 512-519.
   CrossRef    
4. Benzie, I.F.F. and J.J. Strain, 1996. The Ferric Reducing Ability of Plasma (FRAP) as a measure of "antioxidant power": The FRAP assay. Anal. Biochem., 239: 70-76.
   CrossRef    PMid:8660627    Direct Link
5. Brand-Williams, W., M.E. Cuvelier and C. Berset, 1995. Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci. Technol., 28: 25-30.
   CrossRef    
6. Bridle, P. and C.F. Timberlake, 1997. Anthocyanins as natural food colours-selected aspects. Food Chem., 58(1-2): 103-109.
   CrossRef    
7. Cam, M., Y. Hisil and G. Durmaz, 2009. Classification of eight pomegranate juices based on antioxidant capacity measured by four methods. Food Chem., 112: 721-726.
   CrossRef    
8. Cevallos-Casals, B.A. and L. Cisneros-Zevallos, 2004. Stability of anthocyanin-based aqueous extracts for Andean purple corn and red fleshed sweet potato compared to synthetic and natural colorants. Food Chem., 86(1): 69-77.
   CrossRef    
9. Chen, P.N., W.H. Kuo, C.L. Chiang, H.L. Chiou, Y.S. Hsieh and S.C. Chu, 2006. Black rice anthocyanins inhibit cancer cells invasion via repressions of MMPs and u-PA expression. Chem-Biol. Interact., 163: 218-229.
   CrossRef    PMid:16970933    
10. Chen, M.H., S.H. Choi, N. Kozukue, H.J. Kim and M. Friedman, 2012. Growth-inhibitory effects of pigmented rice bran extracts and three red bran fractions against human cancer cells: Relationships with composition and antioxidant activities. J. Agr. Food Chem., 60: 9151-9161.
    CrossRef    PMid:22900641    
11. Cook, K.A., T.E. Dobbs, W.G. Hlady, J.G. Wells, T.J. Barrett, N.D. Puhr, G.A. Lancette, D.W. Bodager, B.L. Toth, C.A. Genese, A.K. Highsmith, K.E. Pilot, L. Finelli and D.L. Swerdlow, 1998. Outbreak of Salmonella serotype Hartford infections associated with unpasteurized orange juice. J. Am. Med. Assoc., 280(17): 1504-1509.
    CrossRef    PMid:9809731    
12. Escribano-Bailon, M.T., C. Santos-Buelga and J.C. Rivas-Gonzalo, 2004. Anthocyanins in cereals. J. Chromatogr. A, 1054: 129-141.
    CrossRef    PMid:15553138    
13. Finocchiaro, F., B. Ferrari, A. Gianinetti, C. Dall'Asta, G. Galaverna, F. Scazzina and N. Pellegrini, 2007. Characterization of antioxidant compounds of red and white rice and changes in total antioxidant capacity during processing. Mol. Nutr. Food Res., 51: 1006-1019.
    CrossRef    PMid:17639995    
14. Gaffa, T., I.A. Jideani and I. Nkama, 2002. Traditional production, consumption and storage of Kunu: A non alcoholic beverage. Plant Foods Hum. Nutr., 57(1): 73-81.
    CrossRef    PMid:11855622    
15. Giusti, M.M. and R.E. Wrolstad, 2005. Characterization and Measurement of Anthocyanins by UV-visible Spectroscopy. In: Wrolstad, R.E. (Ed.), Handbook of Analytical Food Chemistry. John Wiley and Sons, New York, pp: 33-45.
    
16. Gradinaru, G., C.G. Biliaderis, S. Kallithraka, P. Kefalas and C. Garcia-Viguera, 2003. Thermal stability of Hibiscus sabdariffa L. anthocyanins in solution and in solid state: Effects of copigmentation and glass transition. Food Chem., 83(3): 423-436.
    CrossRef    
17. Havlikova, L. and K. Mikova, 1985. Heat stability of anthocyanins. Z. Lebensm. Unters. For., 181: 427-432.
    
18. Hernandez, F., P. Melgarejo, F.A. Tomas-Barberan and F. Artes, 1999. Evolution of juice anthocyanins during ripening of new selected pomegranate (Punica granatum) clones. Eur. Food Res. Technol., 210(1): 39-42.
    CrossRef    
19. Hiemori, M., E. Koh and A.E. Mitchell, 2009. Influence of cooking on anthocyanins in black rice (Oryza sativa L. japonica var. SBR). J. Agr. Food Chem., 57(5): 1908-1914.
    CrossRef    PMid:19256557    
20. Hila, A. and D.O. Castell, 2003. Gastroesophageal reflux disease. Curr. Treat. Option. Gastroenterol., 6(1): 41-48.
    CrossRef    PMid:12521571    
21. Hou, Z.H., P.Y. Qin, Y. Zhang, S.H. Cui and G.X. Ren, 2013. Identification of anthocyanins isolated from black rice (Oryza sativa L.) and their degradation kinetics. Food Res. Int., 50(2): 691-697.
    CrossRef    
22. Hu, C., J. Zawistowski, W.H. Ling and D.D. Kitts, 2003. Black rice (Oryza sativa L. indica) pigmented fraction suppresses both reactive oxygen species and nitric oxide in chemical and biological model systems. J. Agr. Food Chem., 51(18): 5271-5277.
    CrossRef    PMid:12926869    
23. Ivanova, I., P. Kabadjova, A. Pantev, S. Danova and X. Dousset, 2000. Detection, purification and partial characterization of a novel bacteriocin substance produced by Lactococcus lactis susp. lactis B14 isolated from boza Bulgarian traditional cereal beverage. Biocatal. Fundam. Appl., 41: 47-53.
    
24. Jang, S.J. and Z.M. Xu, 2009. Lipophilic and hydrophilic antioxidants and their antioxidant activities in purple rice bran. J. Agr. Food Chem., 57: 858-862.
    CrossRef    PMid:19138081    
25. Kamei, H., Y. Hashimoto, T. Koide, T. Kojima and M. Hasegawa, 1998. Anti-tumor effect of methanol extracts from red and white wines. Cancer Biother. Radio., 13(6): 447-452.
    CrossRef    PMid:10851437    
26. Kasim, N., 2013. Quantitation of antioxidants in Malaysian rice grains. M.Sc. Thesis, Universiti Putra Malaysia, Malaysia.
    
27. Konic-Ristic, A., K. Savikin, G. Zdunic, T. Jankovic, Z. Juranic, N. Menkovic and I. Stankovic, 2011. Biological activity and chemical composition of different berry juices. Food Chem., 125: 1412-1417.
    CrossRef    
28. Ling, W.H., Q.X. Cheng, J. Ma and T. Wang, 2001. Red and black rice decrease atherosclerotic plaque formation and increase antioxidants status in rabbit. J. Nutr., 131: 1421-1426.
    CrossRef    PMid:11340093    
29. Macheix, J.J., A. Fleuriet and J. Billet, 1990. Fruit Phenolics. Importance and Roles of Phenolic Compounds in Fruits. CRC Press, Boca Raton, Fla, pp: 239-293.
    
30. Malien-Aubert, C., O. Dangles and M.J. Amiot, 2001. Color stability of commercial anthocyanin-based extracts in relation to the phenolic composition. Protective effect by intra-and intermolecular copigmentation. J. Agr. Food Chem., 49: 170-176.
    CrossRef    PMid:11170573    
31. Masella, R., C.D. Modesti, C.L. Gennaro and B.E. Coni, 1999. Antioxidant activity of 3,4-DHPEA-EA and protocatechuic acid: A comparative assessment with other olive oil biophenols. Redox Rep., 4: 113-121.
    CrossRef    PMid:10496414     
32. Matthews, S., I. Mila, A. Scalbert and D.M.X. Donnelly, 1997. Extractable and non-extractable proanthocyanidins in barks. Phytochemistry, 45: 405-410.
    CrossRef    
33. Miller, N.J. and C.A. Rice-Evans, 1997. The relative contributions of ascorbic acid and phenolic antioxidants to the total antioxidant activity of orange and apple fruit juices and blackcurrant drink. Food Chem., 60(3): 331-337.
    CrossRef    
34. Mitchell, J.H. and A.R. Collins, 1999. Effects of a soy milk supplement on plasma cholesterol levels and oxidative DNA damage in men: A pilot study. Eur. J. Nutr., 38(3): 143-148.
    CrossRef    PMid:10443336    
35. MLRB (Malaysian Legal Research Board), 2010. Food Act 1983 (Act 281) and Regulations. International Law Book Services. Selangor, Malaysia, pp: 265.
    
36. Morimitsu, Y., K. Kubota, T. Tashiro, E. Hashizume, T. Kamiya and T. Osawa, 2002. Inhibitory effect of anthocyanins and colored rice on diabetic cataract formation in the rat lenses. Int. Congr. Ser., 1245: 503-508.
    CrossRef    
37. Muntana, N. and S. Prasong, 2010. Study on total phenolic contents and their antioxidant activities of Thai white, red and black rice bran extracts. Pak. J. Biol. Sci., 13(4): 170-174.
    CrossRef    PMid:20437683    
38. Nam, S.H., S.P. Choi, M.Y. Kang, H.J. Koh, N. Kozukue and M. Friedman, 2005. Bran extracts from pigmented rice seeds inhibit tumor promotion in lymphoblastoid B cells by phorbol ester. Food Chem. Toxicol., 43(5): 741-745.
    CrossRef    PMid:15778014    
39. Oki, T., M. Masuda, M. Kobayashi, Y. Nishiba, S. Furuta, I. Suda and T. Sato, 2002. Polymeric procyanidins as radical-scavenging components in red-hulled rice. J. Agr. Food Chem., 50: 7524-7529.
    CrossRef    PMid:12475265    
40. Onning, G., B. Akesson, R. Oste and I. Lundquist, 1998. Effects of consumption of oat milk, soya milk, or cow's milk on plasma lipids and antioxidative capacity in healthy subjects. Ann. Nutr. Metab., 42: 211-220.
    CrossRef    PMid:9745107    
41. Ozgen, M., R.N. Reese, A.Z. Tulio Jr., J.C. Scheerens and A.R. Miller, 2006. Modified 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) method to measure antioxidant capacity of selected small fruits and comparison to ferric reducing antioxidant power (FRAP) and 2,2'-diphenyl-1-picrylhydrazyl (DPPH) methods. J. Agr. Food Chem., 54: 1151-1157.
    CrossRef    PMid:16478230    
42. Pedreschi, R. and L. Cisneros-Zevallos, 2007. Phenolic profiles of andean purple corn (Zea mays L.). Food Chem., 100(3): 956-963.
    CrossRef    
43. Porter, L.J., 1993. The Flavonoids: Advances in Research Since 1986. Chapman and Hall, London, pp: 23-55.
    CrossRef    PMid:8425727    
44. Ramos Escudero, F., A.M. Munoz, C. Alvarado-Ortiz, A. Alvarado and J.A. Yanez, 2012. Purple corn (Zea mays L.) phenolic compounds profile and its assessment as an agent against oxidative stress in isolated mouse organs. J. Med. Food, 15(2): 206-215.
    CrossRef    PMid:22082063 PMCid:PMC3264953    
45. Rodrigo, D., A. van Loey and M. Hendrickx, 2007. Combined thermal and high pressure colour degradation of tomato puree and strawberry juice. J. Food Eng., 79(2): 553-560.
    CrossRef    
46. Rohr, G.E., B. Meier and O. Sticher, 2000. Analysis of procyanidins. Stud. Nat. Prod. Chem., 21: 497-537.
    CrossRef    
47. Satoshi, K., T. Madoka and M. Teruyoshi, 2001. Effect of heat and pH on the radical-scavenging activity of proanthocyanidin-rich extract from grape seeds and production of konjac enriched with proanthocyanidin. J. Jpn. Soc. Food Sci. Technol., 48(8): 591-597.
    CrossRef    
48. Scalbert, A., 1992. Quantitative Methods for the Estimation of Tannins in Plant Tissues. In: Hemingway, R.W. and P.E. Laks (Eds.), Plant Polyphenols. Plenum Press, New York, pp: 259-279.
    CrossRef    
49. Shams-Ardekani, M.R., M. Hajimahmoodi, M.R. Oveisi, N. Sadeghi, B. Jannat, A. Mohammad Ranjbar, N. Gholam and T. Moridi, 2011. Comparative antioxidant activity and total flavonoid content of Persian pomegranate (Punica granatum L.) cultivars. Iran. J. Pharm. Res., 10(3): 519-524.
    PMid:24250384 PMCid:PMC3813023    
50. Singleton, V.L., R. Orthofer and R.M. Lamuela-Raventos, 1999. Analysis of total phenols and other oxidation subtrates and antioxidants by means of Folin-Ciocalteu reagent. Method. Enzymol., 299: 152-175.
    CrossRef    
51. Slimestad, R. and H. Solheim, 2002. Anthocyanins from black currants (Riber nigrum L.). J. Agr. Food Chem., 50: 3228-3231.
    CrossRef    
52. Slinkard, K. and V.L. Singleton, 1977. Total phenols analysis: Automation and comparison with manual methods. Am. J. Enol. Viticult., 28: 49-55.
    
53. Sompong, R., S. Siebenhandl-Ehn, G. Linsberger-Martin and E. Berghofer, 2011. Physicochemical and antioxidative properties of red and black rice varieties from Thailand, China and Sri Lanka. Food Chem., 124: 132-140.
    CrossRef    
54. Stannard, C., 1997. Development and use of microbiological criteria for foods. Food Sci. Technol. Today, 11(3): 137-176.
    
55. Sun, T., J.R. Powers and J. Tang, 2007. Evaluation of the antioxidant activity of asparagus, broccoli and their juices. Food Chem., 105: 101-106.
    CrossRef    
56. Thaipong, K., U. Boonprakob, K. Crosby, L. Cisneros-Zevallos and D.H. Byrne, 2006. Comparison of ABTS, DPPH, FRAP and ORAC assays for estimating antioxidant activity from guava fruit extracts. J. Food Compos. Anal., 19: 669-675.
    CrossRef    
57. Todorov, S.D. and L.M.T. Dicks, 2005. Pediocin ST18: An anti-listerial bacteriocin produced by Pediococcus pentosaceus ST18 isolated from boza, a traditional cereal beverage from Bulgaria. Proc. Biochem., 40(1): 365-370.
    CrossRef    
58. Torskangerpoll, K. and O.M. Andersen, 2005. Colour stability of anthocyanins in aqueous solutions at various pH values. Food Chem., 89: 427-440.
    CrossRef    
59. Tseng, T.H., C.J. Wang, E.S. Kao and H.Y. Chu, 1996. Hibiscus protocatechuic acid protects against oxidative damage induced by tert-butylhydroperoxide in rat primary hepatocytes. Chem. Biol. Interact., 101(2): 137-148.
    CrossRef    
60. Vanderzant, C., 1992. Compendium of Methods for the Microbiological Examination of Foods. 3rd Edn., American Public Health Association, Washington, D.C.
    PMid:22059567    
61. West, N.X., J.A. Hughes, D.M. Parker, R.G. Newcombe and M. Addy, 1999. Development and evaluation of a low erosive blackcurrant juice drink 2. Comparison with a conventional blackcurrant juice drink and orange juice. J. Dent., 27(5): 341-344.
    CrossRef    
62. Xia, M., W.H. Ling, J. Ma, D.D. Kitts and J. Zawistowski, 2003. Supplementation of diets with black rice pigment fraction attenuates atherosclerotic plaque formation in apolipoprotein E deficient mice. J. Nutr., 133: 744-751.
    CrossRef    PMid:12612147    
63. Xu, Z., N. Hua and J.S. Godber, 2001. Antioxidant activity of tocopherols, tocotrienols and ?-oryzanol components from rice bran against cholesterol oxidation accelerated by 2,2'-azobis (2-methylpropionamidine) dihydrochloride. J. Agr. Food Chem., 49: 2077-2081.
    CrossRef    PMid:11308370    
64. Yin, M.C., C.C. Lin, H.C. Wu, S.M. Tsao and C.K. Hsu, 2009. Apoptotic effects of protocatechuic acid in human breast, lung, liver, cervix and prostate cancer cells: Potential mechanisms of action. J. Agr. Food Chem., 57: 6468-6473.
    CrossRef    PMid:19601677    
65. Yoshinaga, K., K. Takahashi and K. Yoshizawa, 1986. Liqueur making using pigments of red rice. J. Brew. Soc. Jpn., 75 (5): 337-340.
    CrossRef    

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