Abstract

In order to obtain the optimum inoculum size of goat milk fermented by probiotics, the total inoculum size containing L. acidophilus or L. casei on pH, acidity and viable counts and sensory during fermentation were studied on the basis of S. thermophilus and L. bulgaricus as starter cultures. The results showed as follows: the optimum inoculum size of L. acidophilus and L. casei were all 7% and goat milk was fermented at 39°C for 4.5 h. The pH, acidity, the viable counts of L. acidophilus and the total viable counts were respectively 4.48, 91°T, 1.60×10⁷ cfu/mL and 1.69×109 cfu/mL. The pH, acidity, the viable counts of L. casei and the total viable counts were respectively 4.38, 96°T, 2.80×10⁸ cfu/mL and 2.20×10⁹ cfu/mL.

Keywords:

Goat milk, inoculum size, L. acidophilus, L. casei, yogurt,

References

1. Agnihotri, M.K. and V.S.S. Prasad, 1993. Biochemistry and processing of goat milk and milk products. Small Ruminant Res., 12: 151-170.
   CrossRef    
2. Betoret, N., L. Puente, M.J. Diaz, M.J. Pagan, M.J. Garcia and M.L. Gras, 2003. Development of robiotic-enriched dried fruits by vacuum impregnation. Food Eng., 56(2-3): 273-277.
   CrossRef    
3. Chen, H., C.F. Wang, G.W. Shu, D. Peng and J.J. Zhang, 2010. Technological optimization of set-style goat yogurt fermentation. Food Sci. Technol., 35(12): 71-74.
   
4. Gao, S.B., 2004. The latest progress of fermented milk. J. China. Dairy Ind., 32(8): 46-49.
   
5. Golden, B.R., 1998. Health benefits of probiotic. Brit. J. Nutr., 80: 203-207.
   
6. Haenlein, G.F.W., 2004. Goat milk in human nutrition. Small Ruminant Res., 51: 155-163.
   CrossRef    
7. Jack, R.W., J.R. Tagg and B. Ray, 1995. Bacteriocins of gram-positive bacteria. Microbial. Rev., 59(1): 171-200.
   PMid:7603408 PMCid:PMC239359    Direct Link
8. Kashket, E.R., 1985. The proton motive force in bacteria: A critical assessment of methods. Annu. Rev. Microbiol., 39: 219-242.
   CrossRef    PMid:2998266    
9. Keogh, M.K. and B.T. O'Kennedy, 1998. Rheology of stirred yogurt as affected by added milk fat, protein and hydrocolloids. J. Food Sci., 63: 108-112.
   CrossRef    
10. Lina, J.B., D.G. Dianiel and F.B. Frank, 2000. The role of probiotic cultures in prevention of colon. J. Nutr., 130: 410-414.
    
11. Rafter, J., 2003. Probiotics and colon cancer. Best Pract. Res. Cl. Ga., 17(5): 849-859.
    CrossRef    
12. Rial, D.R., 2000. The role of probiotic cultures in the control of gastrointestinal health. J. Nutr., 130: 396-402.
    
13. Saarela, M., L. Lähteenäki, R. Crittenden, S. Salminen and T. Mattila-Sandholm, 2002. Gut bacteria and health foods-the European perspective. J. Food Microbiol., 78: 99-117.
    CrossRef    
14. Shu, G.W., Z.X. Ma, Z.W. Wang and H. Chen, 2011. Effect of bile and nalidixic acid on growth of selected probiotics. Adv. Mater. Res., 322: 248-251.
    CrossRef    
15. Shu, G.W., C. Li, H. Chen and C.F. Wang, 2014. Effect of inoculum and temperature on the fermentation of goat yogurt. Adv. J. Food Sci. Technol., 6(1): 68-71.
    CrossRef    
16. Tharmaraj, N. and N.P. Shah, 2000. Probiotic bacteria: Selective enumeration and survival in dairy foods. J. Dairy Sci., 83(4): 894-907.
    CrossRef    
17. Vinderola, C.G., N. Bailo and J.A. Reinheimer, 2002. Interactions among lactic acid starter probiotic bacteria used for fermented dairy products. J. Dairy Sci., 85: 721-729.
    CrossRef    

Competing interests

The authors have no competing interests.

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