Abstract

Phosphatidylinositol (PI) was extracted from soybean phospholipid using alcohol-n-hexane. The optimum extraction conditions were 56% ethanol, the ethanol and n-hexane (1.7:1, by volume), 0.3 g/L Ca²⁺ and pH = 8.17 with ammonia solution in 56% ethanol. The purity of PI in soybean phospholipid was increased from 29.00 to 84.17% under the optimum condition.

Keywords:

Fractionation degree, phosphatidylionsitol, purification, soybean phospholipid,

References

1. Berridge, M.J., 1984. Inositol trisphosphate and diacylglycerol as second messengers. Biochem. J., 220: 345-360.
   CrossRef    PMid:6146314 PMCid:PMC1153635    
2. Chen, Z., H. An and X. He, 2005. The Fractionation of Phosphatidylinositol with ethanol. Qiqihar Univ., 21: 1-4.
   
3. Wang, J., S. Zhu and C. Xue, 2002. Biochemistry. Beijing, 9: 103.
   
4. Wu, P., H. An, X. He and C. Qiao, 2006. Fractionation of phosphatidylinositol from soybean phospholipid with alcohol-n-hexane. China Oil. Fat., 31: 87-89.
   

Competing interests

The authors have no competing interests.

Open Access Policy

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Copyright

The authors have no competing interests.