Home            Contact us            FAQs
    
      Journal Home      |      Aim & Scope     |     Author(s) Information      |      Editorial Board      |      MSP Download Statistics

     Research Journal of Applied Sciences, Engineering and Technology


Encapsulation Red Ginger Oleoresin (Zingiber officinale var. Rubrum) With Chitosan-alginate as Wall Material Using Spray Drying

1Jayanudin, 2Rochmadi, 2Wiratni, 1Meri Yulvianti, 1Dhena Ria Barleany and 1Widya Ernayati
1Department of Chemical Engineering, Faculty Engineering, Sultan Ageng Tirtayasa University Jl. Jenderal Sudirman km.03 Cilegon 42435, Indonesia
2Department of Chemical Engineering, Faculty Engineering, Gajah Mada University Jl. Grafika, No. 2, Yogyakarta 55281, Indonesia
Research Journal of Applied Sciences, Engineering and Technology  2015  12:1370-1378
http://dx.doi.org/10.19026/rjaset.10.1836  |  © The Author(s) 2015
Received: March ‎3, ‎2015  |  Accepted: March ‎14, ‎2015  |  Published: August 25, 2015

Abstract

Encapsulation is the process of protecting the active ingredients that is susceptible to environmental influences by using a coating. Red ginger oleoresin contains bioactive components that can be used as natural antioxidants, but sensitive to environmental influences. Chitosan-alginate nanoparticle is used as the coating, because it is safe for consumption and also stable. The purpose of this research was to determine the effect of the Tripolyphosphate (TPP) concentration against the emulsion droplet size and determine the encapsulation efficiency of red ginger oleoresin. Encapsulation method was done by mixing 2% chitosan solution with 1% acetic acid, 1% sodium alginate and 8 g of red ginger oleoresin. The mixture was stirred and added sodium tripolyphosphate (3.5, 4.5 and 5.5%, respectively), emulsion preparation process was done by adding tween 80 (3, 4 and 5%, respectively) and then stirred using homogenizer with a speed of 22,000 rpm, emulsions formed were analyzed using nano-particle analyzer. Emulsion formed was flowed on to the spray dryer inlet temperature of 180οC to form a powder encapsulation. Powder products were analyzed for determining encapsulation efficiency and morphology of red ginger oleoresin powder using Scanning Electron Microscopy (SEM). Based on the research results, the smallest droplet size of the emulsion was obtained at 481.5 nm and the largest encapsulation efficiency was as high as 70.59%.

Keywords:

Chitosan , encapsulation , red ginger oleoresin , sodium alginate , tripolyphosphate,


References

  1. Aghbashlo, M., H. Mobli, A. Madadlou and S. Rafiee, 2012. Influence of wall material and inlet drying air temperature on the microencapsulation of fish oil by spray drying. Food Bioprocess Tech., 6(6): 1347-1356.
  2. Ali, B.H., G. Blunden, M.O. Tanira and A. Nemmar, 2008. Some phytochemical, pharmacological and toxicological properties of ginger (Zingiber officinale Roscoe): A review of recent research. Food Chem. Toxicol., 46(2): 409-420.
    CrossRef    PMid:17950516    
  3. Al-Qadi, S., A. Grenha, D. Carrión-Recio, B. Seijo and C. Remuńán-López, 2012. Microencapsulated chitosan nanoparticles for pulmonary protein delivery: In vivo evaluation of insulin-loaded formulations. J. Control. Release, 157(3): 383-390.
    CrossRef    PMid:21864592    
  4. Anekella, K and V. Orsat, 2013. Optimization of microencapsulation of probiotics in raspberry juice by spray drying. LWT-Food Sci. Technol., 50(1): 17-24.
  5. Aral, C. and J. Akbuga, 1998. Alternative approach to the preparation of chitosan beads. Int. J. Pharm., 168(1): 9-15.
    CrossRef    
  6. Bae, E.K. and S.J. Lee, 2008. Microencapsulation of avocado oil by spray drying using whey protein and maltodextrin. J. Microencapsul., 25(8): 549-560.
    CrossRef    PMid:18465295    
  7. Balasubramani, P., R. Viswanathan and M. Vairamani, 2013. Response surface optimisation of process variables for microencapsulation of garlic (Allium sativum L.) oleoresin by spray drying. Biosyst. Eng., 114(3): 205-213.
    CrossRef    
  8. Bansode, S.S., S.K. Banarjee, S.L. Gaikwad, R. Jadhav and R.M. Thorat, 2010. Microencapsulation: A review. Int. J. Pharm. Sci. Rev. Res., 1(1): 38-43.
  9. Bellik, Y., 2014. Total antioxidant activity and antimicrobial potency of the essential oil and oleoresin of Zingiber officinale roscoe. Asian Pac. J. Trop. Dis., 4(1): 40-44.
    CrossRef    
  10. Bhattarai, S., V.H. Tran and C.C. Duke, 2007. Stability of [6]-gingerol and [6]-shogaol in simulated gastric and intestinal fluids. J. Pharmaceut. Biomed., 45: 648-653.
    CrossRef    PMid:17706909    
  11. Borrmanna, D., A.P.T.R. Pierucci., S.G.F. Leite and M.H.M.R. Leao, 2013. Microencapsulation of passion fruit (Passiflora) juice with n-octenylsuccinate-derivatised starch using spray-drying. Food Bioprod. Process., 91(1): 23-27.
    CrossRef    
  12. Bosio, V.E., S. Basu, F. Abdullha, M.E.C. Villalba, J.A. Guida, M. Arup and G.R. Castro, 2014. Encapsulation of Congo red in carboxymethyl guar gum-alginate gel microspheres. React. Funct. Polym., 82: 103-110.
    CrossRef    
  13. Calvo, P., T. Hernández, M. Lozano and D.G. Gómez. 2010. Microencapsulation of extra-virgin olive oil by spray-drying: Influence of wall material and olive quality. Eur. J. Lipid Sci. Tech., 112(8): 852-858.
    CrossRef    
  14. Carvalho, A.G.S., V.M. Silva and M.D. Hubinger, 2013. Microencapsulation by spray drying of emulsified green coffee oil with two-layered membranes. Food Res. Int., 61: 236-245.
    CrossRef    
  15. Choi, J.S., Y. Kim, J. Kang, S.Y. Jeong and H.S.Yoo, 2013. Electrospun chitosan microspheres for complete encapsulation of anionic proteins: Controlling particle size and encapsulation efficiency. AAPS PharmSciTech., 14(2): 794-801.
    CrossRef    PMid:23636817 PMCid:PMC3665992    
  16. Chrubasik, M.H., B.D. Pittlerc and Roufogalis, 2005. Zingiberis rhizoma: A comprehensive review on the ginger effect and efficacy profiles. Phytomedicine, 12(9): 684-701.
    CrossRef    PMid:16194058    
  17. Cook, M.T., G. Tzortzis, D. Charalampopoulos, V. Vitaliy and V.V. Khutoryanskiy, 2014. Microencapsulation of a synbiotic into PLGA/alginate multiparticulate gels. Int. J. Pharm., 466(1-2): 400-408.
    CrossRef    PMid:24657143    
  18. Dubey, R., T.C. Shami and K.U.B. Rao, 2009. Microencapsulation technology and applications. Defence Sci. J., 59(1): 82-95.
  19. Dugasani, S., M.R. Pichikac, V.D. Nadarajah, M.K. Balijepalli, S. Tandra and J.N. Korlakunta, 2010. Comparative antioxidant and anti-inflammatory effects of [6]-gingerol, [8]-gingerol, [10]-gingerol and [6]-shogaol. J. Ethnopharmacol., 127(2): 515-520.
    CrossRef    PMid:19833188    
  20. Eid, A.M.M., S.H. Baie and O.M. Arafat, 2012. The effect of surfactant blends on the production of self- emulsifying system. Int. J. Pharm. Frontier Res., 2(2): 21-31.
  21. El-Aassara, M.R., E.E. Hafezb, N.M. El-Deebc and M.M.G. Fouda, 2014. Microencapsulation of lectin anti-cancer agent and controlled releaseby alginate beads, biosafety approach. Int. J. Biol. Macromol., 69: 88-94.
    CrossRef    PMid:24857870    
  22. El-Ghorab, A.H., M. Nauman, F.M. Anjum, S. Hussain and M. Nadeem, 2010. A comparative study on chemical composition and antioxidant activity of ginger (Zingiber officinale) and Cumin (Cuminum cyminum). J. Agr. Food Chem., 58(14): 8231-8237.
    CrossRef    PMid:20590154    
  23. Evans, R.C., N.J. Miller and G. Paganga, 1996. Structure-antioxidant activity relationships of flavonids and phenolic acids. Free Radical Bio. Med., 20(7): 933-956.
    CrossRef    
  24. Eze, J.I. and K.E. Agbo, 2011. Comparative studies of sun and solar drying of peeled and unpeeled ginger. Am. J. Sci. Ind. Res., 2(2): 136-143.
    CrossRef    
  25. Ezhilarasi, P.N., P. Karthik, N. Chhanwal and C. Anandharamakrishnan, 2013. Nan encapsulation techniques for food bioactive components: A review. Food Bioprocess Tech., 6(3): 628-647.
    CrossRef    
  26. Friedli, A.C. and I.R. Schlager, 2005. Demonstrating encapsulation and release: A new take on alginate complexation and the nylon rope trick. J. Chem. Educ., 82(7): 1017-1020.
    CrossRef    
  27. Gallardo, G., L. Guida, V. Martinez, M.C. López, D. Bernhardt, R. Blasco, R.P. Islas and L.G. Hermida, 2013. Microencapsulation of linseed oil by spray drying for functional food application. Food Res. Int., 52(2): 473-482.
    CrossRef    
  28. Gouin, S., 2004. Microencapsulation: Industrial appraisal of existing technologies and trends. Trends Food Sci. Tech., 15(7-8): 330-347.
    CrossRef    
  29. Goycoolea, F.M., G. Lollo, C.R. Lo´pez, F. Quaglia and M.J. Alonso, 2009. Chitosan-alginate blended nanoparticles as carriers for the transmucosal delivery of macromolecules. Biomacromolecules, 10(7): 1736-1743.
    CrossRef    PMid:19545119    
  30. Hosseini, S.F., M. Zandi, M. Rezaei and F. Farahmandghavi, 2013. Two-step method for encapsulation of oregano essential oil in chitosan nanoparticles: Preparation, characterization and in vitro release study. Carbohyd. Polymers, 95(1): 50-56.
    CrossRef    PMid:23618238    
  31. Jafari, S.M., E. Assadpoor, Y. He and B. Bhandari, 2008. Encapsulation efficiency of food flavours and oils during spray drying. Dry. Technol., 26(7): 816-835.
    CrossRef    
  32. Jarudilokkul, S., A. Tongthammachat and V. Boonamnuayvittaya, 2011. Preparation of chitosan nanoparticles for encapsulation and release of protein. Korean J. Chem. Eng., 28(5): 1247-1251.
    CrossRef    
  33. Jayanudin, R.B.D., Rochmadi, Wiratni, A. Sugiarti and Y.A. Kusuma, 2013. Modification on maceration extraction to the yield and components of red ginger oleoresin. Proceeding of International Conference on Chemical Engineering. Parahyangan University, pp: 72-77.
  34. Jonassen, H., A.L. Kjřniksen and M. Hiorth, 2012. Stability of chitosan nanoparticles cross-linked with tripolyphosphate. Biomacromolecules, 13(11): 3747-3756.
    CrossRef    PMid:23046433    
  35. Kha, T.C., M.H. Nguyen, P.D. Roach and C.E. Stathopoulos, 2014. Microencapsulation of Gac oil: Optimisation of spray drying conditions using response surface methodology. Powder Technol., 264: 298-309.
    CrossRef    
  36. Kiran, C.R., A.K. Chakka, K.P.P. Amma, A.N. Menon, M.M.S. Kumar and V.V. Venugopalan, 2013. Influence of cultivar and maturity at harvest on the essential oil composition, oleoresin and [6]-Gingerol contents in fresh ginger from northeast India. J. Agr. Food Chem., 61(17): 4145-4154.
    CrossRef    PMid:23570262    
  37. Kizhakkayil, J. and B. Sasikumar, 2012. Characterization of ginger (Zingiber officinale Rosc.) germplasm based on volatile and non-volatile components. Afr. J. Biotechnol., 11(4): 777-786.
    CrossRef    
  38. Klaypradit, W. and Y.W. Huang, 2008. Fish oil encapsulation with chitosan using ultrasonic atomizer. LWT-Food Sci. Technol., 41(6): 1133-1139.
  39. Koppolu, B.P., S.G. Smith, S. Ravindranathan, S. Jayanthi, T.K.S. Kumar and D.A. Zaharoff, 2014. Controlling chitosan-based encapsulation for protein and vaccine delivery. Biomaterials, 35(14): 4382-4389.
    CrossRef    PMid:24560459 PMCid:PMC3975584    
  40. Liu, X.D., T.T. Furuta, H. Yoshii and P. Linko, 2000. Retention of emulsified flavor in a single droplet during drying. Food Sci. Technol. Res., 6: 335-339.
    CrossRef    
  41. Liu, X.D., T. Atarashi, T. Furuta, H. Yoshii, S. Aishima and M. Ohkawara, 2001. Microencapsulation of emulsified hydrophobic flavors by spray drying. Dry. Technol., 19(7): 1361-1374.
    CrossRef    
  42. Lun, L.C., T. Derong and L. Le, 2008. Research on the extracting and anti-oxidation dynamic characteristics of ginger oleoresin. Int. J. Food Sci. Tech., 43(3): 517-525.
    CrossRef    
  43. Maciel, G.M., K.S. Chaves, C.R.F. Grosso and M.L. Gigante, 2014. Microencapsulation of Lactobacillus acidophilus La-5 by spray-drying using sweet whey and skim milk as encapsulating materials. J. Dairy Sci., 97(4): 1991-1998.
    CrossRef    
  44. McClements, D.J., 2011. Edible nanoemulsions: Fabrication, properties and functional performance. Soft Matter, 7(6): 2297-2316.
    CrossRef    
  45. Nadeem, H.S., C. Dinçer, M. Torun, A. Topuz and F. Özdemir, 2013. Influence of inlet air temperature and carrier material on the production of instant soluble sage (Salvia fruticosa Miller) by spray drying. LWT-Food Sci. Technol., 52(1): 31-38.
  46. Nwaoha, M., I. Elizabeth, Okafor, G. Ifeanyi and A.O. Veronica, 2013. Production of oleoresin from ginger (Zingiber officinale) peels and evaluation of its antimicrobial and antioxidative properties. Afr. J. Microbiol. Res., 7(42): 4981-4989.
    CrossRef    
  47. Oboh, G., J.A. Ayodele and O.A. Adedayo, 2012. Antioxidant and inhibitory effect of red ginger (Zingiber officinale var. Rubra) and white ginger (Zingiber officinale Roscoe) on Fe2+ induced lipid peroxidation in rat brain in vitro. Exp. Toxicol. Pathol., 64(1-2): 31-36.
    CrossRef    PMid:20598871    
  48. Oliveira, E.F., H.C.B. Paulaa and R.C.M. de Paula, 2014. Alginate/cashew gum nanoparticles for essential oil encapsulation. Colloid. Surface. B, 113: 146-151.
    CrossRef    PMid:24077112    
  49. Onyenekwe, P.C., 2000. Assessment of oleoresin and gingerol contents in gamma irradiated ginger rhizomes. Mol. Nutr. Food Res., 44(2): 130-132.
    CrossRef    
  50. Pawar, N., S. Pai, M. Nimbalkar and G. Dixit, 2011. RP-HPLC analysis of phenolic antioxidant compound 6-gingerol from different ginger cultivars. Food Chem., 126(3): 1330-1336.
    CrossRef    
  51. Picone, C.S.F. and R.L. Cunha, 2013. Formation of nano and microstructures by polysorbate-chitosan association. Colloid. Surface. A, 418: 29-38.
    CrossRef    
  52. Saberi, M., M. Hashemiravan and N. Farhadyar, 2014. Influence of casein and inulin on the properties of nano-particle encapsulation of fish oil. J. Biodiversity Environ. Sci., 4(2): 318-326.
  53. Santa-Maria, M., H. Scher and T. Jeoh, 2012. Microencapsulation of bioactives in cross-linked alginate matrices by spray drying. J. Microencapsul., 29(3): 286-295.
    CrossRef    PMid:22251237    
  54. Sathyabama, S., M.R. Kumar, P.B. Devi, R. Vijayabharathi and V.B. Priyadharisini, 2014. Co-encapsulation of probiotics with prebiotics on alginate matrix and its effect on viability in simulated gastric environment. LWT-Food Sci. Technol., 57(1): 419-425.
  55. Schatz, C., A. Domard, C. Viton, C. Pichot and T. Delair, 2004. Versatile and efficient formation of colloids of biopolymer-based polyelectrolyte complexes. Biomacromolecules, 5(5): 1882-1892.
    CrossRef    PMid:15360302    
  56. Schutz, C.A., L.J. Jeanneret, P. Ka¨uper and C. Wandrey, 2011. Cell response to the exposure to chitosan-TPP//alginate nanogels. Biomacromolecules, 12: 4153-4161.
  57. Shaikh, J., R. Bhosale and R. Singhal, 2006. Microencapsulation of black pepper oleoresin. Food Chem., 94(1): 105-110.
    CrossRef    
  58. Shu, X.Z. and K.J. Zhu, 2000. A novel approach to prepare tripolyphosphate: Chitosan complex beads for controlled release drug delivery. Int. J. Pharm., 201(1): 51-58.
    CrossRef    
  59. Shu, X.Z. and K.J. Zhu, 2002. Controlled drug release properties of ionically cross-linked chitosan beads: The influence of anion structure. Int. J. Pharm., 233: (1-2): 217-225.
    CrossRef    
  60. Singh, G., I.P.S. Kapoor, P. Singh, C.S. de Heluani, M.P. de Lampasona and C.A.N. Catalan, 2008. Chemistry, antioxidant and antimicrobial investigations on essential oil and oleoresins of Zingiber officinale. Food Chem. Toxicol., 46(10): 3295-3302.
    CrossRef    PMid:18706468    
  61. Sivasothy, Y., W.K. Chong, A. Hamid, I.M. Eldeen, S.F. Sulaiman and K. Awang, 2011. Essential oils of Zingiber officinale var. rubrum Theilade and their antibacterial activities. Food Chem., 124(2): 514-517.
    CrossRef    
  62. Soottitantawat, A., H. Yoshii, T. Furuta, M. Ohkawara and P. Linko, 2003. Microencapsulation by spray drying: Influence of emulsion size on the retention of volatile compounds. J. Food Sci., 68(7): 2256-2262.
    CrossRef    
  63. Soottitantawat, A., F. Bigeard, H. Yoshii, T. Furuta, M. Ohkawara and P. Linko, 2005. Influence of emulsion and powder size on the stability of encapsulated D-limonene by spray drying. Innov. Food Sci. Emerg., 6(1): 107-114.
    CrossRef    
  64. Tan, L.H., L.W. Chan and P.W.S. Heng, 2005. Effect of oil loading on microspheres produced by spray drying. J. Microencapsul., 22(3): 253-259.
    CrossRef    PMid:16019911    
  65. Torres, L.M., E.E. García-Cruz, F. Calderas, R.F.G. Laredo, G.S. Olivares, J.A.G. Infante, N.E.R. Guzmán and J.R. Ramírez, 2013. Microencapsulation by spray drying of gallic acid with nopal mucilage (Opuntia ficus indica). LWT-Food Sci. Technol., 50(2): 642-650.
    Direct Link
  66. Trifkovic, K.T., N.Z. Milasinovic, V.B. Djordjevic, M.T.K. Krusic, D. Zorica, K. Jugovic, V.A. Nedovic and B.M. Bugarskia, 2014. Chitosan microbeads for encapsulation of thyme (Thymus serpyllum L.) polyphenols. Carbohyd. Polym., 111: 901-907.
    CrossRef    PMid:25037430    
  67. Vaidya, S., R. Bhosale and R.S. Singhal, 2006. Microencapsulation of cinnamon oleoresin by spray drying using different wall materials. Dry. Technol., 24(8): 983-992.
    CrossRef    
  68. Venkatesan, P., R. Manavalan and K. Valliappan, 2009. Microencapsulation: A vital technique in novel drug delivery system. J. Pharm. Sci. Res., 1(4): 26-32.
  69. Wittayasuporn, M., S. Rengpipat, T. Palaga, P. Asawanonda, N. Anumansirikul and S.P. Wanichwecharungruang, 2010. Chitosan derivative nanocarrier: Safety evaluation, antibacterial property and ascorbyl palmitate encapsulation. J. Microencapsul., 27(3): 218-225.
    CrossRef    PMid:19566395    
  70. Wohlmuth, H., D.N. Leach, M.K. Smith and S. Myers, 2005. Gingerol content of diploid and tetraploid clones of ginger (Zingiber officinale Roscoe). J. Agr. Food Chem., 53(14): 5772-5778.
    CrossRef    PMid:15998147    
  71. Yeh, H.Y., C.H. Chuang, H.C. Chen, C.J. Wan, T.L. Chen and L.Y. Lin, 2014. Bioactive components analysis of two various gingers (Zingiber officinale Roscoe) and antioxidant effect of ginger extracts. LWT-Food Sci. Technol., 55(1): 329-334.
  72. Zarate, R. and R.R. Yeoman, 1996. Changes in the amounts of [6] gingerol and derivatives during a culture cycle of ginger, Zingiber officinale. Plant Sci., 121(1): 115-122.
    CrossRef    

Competing interests

The authors have no competing interests.

Open Access Policy

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Copyright

The authors have no competing interests.

ISSN (Online):  2040-7467
ISSN (Print):   2040-7459
Submit Manuscript
   Information
   Sales & Services
Home   |  Contact us   |  About us   |  Privacy Policy
Copyright © 2024. MAXWELL Scientific Publication Corp., All rights reserved