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     Research Journal of Applied Sciences, Engineering and Technology


Bioinformatics Analysis of the Duck Enteritis Virus UL54 Gene

1, 3Chaoyue Liu, 1, 2, 3Anchun Cheng and 1, 2, 3Mingshu Wang
1Institute of Preventive Veterinary Medicine
2Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan, 611130, P.R. China
3Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, 46 Xinkang Road, Ya
Research Journal of Applied Sciences, Engineering and Technology  2014  14:2813-2817
http://dx.doi.org/10.19026/rjaset.7.604  |  © The Author(s) 2014
Received: October 31, 2012  |  Accepted: January 03, 2013  |  Published: April 12, 2014

Abstract

In this study, we analyze the Duck Enteritis Virus (DEV) UL54 gene, which has been isolated and identified in our lab (GenBank accession NO EU071033), to help deeply research on DEV. DNA sequence analysis showed that the identified ORF which composed of 1377 bp nucleotides encoded 458 amino acids with a predicted Mr. of 51.75 kDa. Multiple sequence alignment suggested that the UL54 gene was highly conserved in Alphaherpesvirinae and was similar to the other herpesviral UL54 gene. Phylogenetic analysis of the DEV UL54 gene revealed that DEV had a close evolutionary relationship with Gallid, Herpesvirus 2 (GaHV-2), Gallid Herpesvirus 3 (GaHV-3), Meleagrid Herpesvirus1 (MeHV-1) and should belong to a single cluster within the Alphaherpesvirinae subfamily.

Keywords:

DEV, molecular characterization, sequence analysis, UL54,


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Competing interests

The authors have no competing interests.

Open Access Policy

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Copyright

The authors have no competing interests.

ISSN (Online):  2040-7467
ISSN (Print):   2040-7459
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