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2016 (Vol. 8, Issue: 4)
Research Article

Isolation and Molecular Identification of New Benzene Degrading Lysinibacillus Strains from Gasoline Contaminated Soil

Fawzi I. Irshaid and Jacob H. Jacob
Department of Biological Sciences, Faculty of Science, Al Al-Bayt University, P.O. Box 130040, Al-Mafraq 25113, Jordan

DOI: 10.19026/rjees.8.3064
Submitted Accepted Published
January ‎25, ‎2016 April ‎22, ‎2016 November 20, 2016

  How to Cite this Article:

Fawzi I. Irshaid and Jacob H. Jacob, 2016. Isolation and Molecular Identification of New Benzene Degrading Lysinibacillus Strains from Gasoline Contaminated Soil.  Research Journal of Environmental and Earth Sciences, 8(4): 34-43.

DOI: 10.19026/rjees.8.3064

URL: http://www.maxwellsci.com/jp/mspabstract.php?jid=RJEES&doi=rjees.8.3064


The aim of this study was to isolate and identify aerobic benzene-degrading bacteria from soil sample contaminated with gasoline. Soil samples were collected from gasoline-contaminated sites located around gas stations in the Al-Mafraq city of Jordan. Ten grams of soil were transferred to Stanier's medium supplemented with 1% benzene and aerobically incubate at 30C for 72 h. Five morphologically different bacterial isolates were recovered from soil samples and designated as 1J1, 2J2, DJ2, 3J4 and 1J1-2. These isolates were subjected to analyses for identification and characterization. All isolates were Gram positive, endospore-forming, motile, mesophilic and rod-shaped aerobic bacteria. Biochemically, all were catalase and oxidase positive except DJ2, which was negative for oxidase. Based on their 16S rDNA sequences, they appear to be closely related to the genus Lysinibacillus (L). Isolate 1J1 revealed 91% homology with L. fusiformis, suggesting a member of a new genus with some similarity to the genus Lysinibacillus. Isolates 2J2, DJ2, 3J4 and 1J1-2 exhibited more than 97% sequence identity to L. xylanilyticus, L. odeysseyi, L. macrolides and L. boronitolerans, respectively. The 16S rDNA sequences of these isolates were deposited in GenBank database. All isolates grew best in Stanier's medium when the optimal growth conditions were 1% benzene, 30C and pH 6.8. In conclusion, five novel bacterial isolates with similarity to the genus Lysinibacillus that utilize benzene as the sole carbon and energy source were isolated and characterized from soil samples contaminated by gasoline. These five isolates can be considered as ideal candidates for bioremediation of benzene from soils.

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    Competing interests

The authors have no competing interests.
    Open Access Policy

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.


© The Author(s) 2016

ISSN (Online):  2041-0492
ISSN (Print):   2041-0484
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