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     Advance Journal of Food Science and Technology


Antioxidant Activity in vitro and in vivo of Auricularia auricula Polysaccharides through Different Extraction Processes

Siqi Xu and Yongjun Zhang
College of Life Science, China Jiliang University, 258 Xueyuan Street, Hangzhou 310018, Zhejiang, China
Advance Journal of Food Science and Technology  2016  9:485-497
http://dx.doi.org/10.19026/ajfst.12.3059  |  © The Author(s) 2016
Received: November ‎30, ‎2015  |  Accepted: February ‎23, ‎2016  |  Published: November 25, 2016

Abstract

In this study, antioxidant effects in vitro and in vivo by the Caenorhabditis elegans model of Auricularia auricula polysaccharides (AAP1, AAP2 and AAP3) were used to compare these three kinds of polysaccharide extraction processes (hot water extraction, enzyme extraction and alkali extraction). And the monosaccharide composition of these polysaccharides was determined by Gas Chromatography (GC) method. Results suggested that AAP2, which was mainly composed of arabinose, D-xylose, 2-Deoxy-D-arabino-hexose, mannose, glucose and galactose with the molar ratio of 1:0.44:0.33:1.67:1:0.17, possessed stronger antioxidant activity. It could scavenge free radicals, up-regulation of antioxidant enzyme (superoxide dismutase (SOD) by 91.25% and catalase (CAT) by 36.67%, compared with control). It could also reduce the level of Reactive Oxygen Species (ROS) in C. elegans under oxidative stress. In conclusion, enzyme extraction is better in preparing AAP.

Keywords:

Auriculaia auricula polysaccharide, antioxidant activity, extraction processes,


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Competing interests

The authors have no competing interests.

Open Access Policy

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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The authors have no competing interests.

ISSN (Online):  2042-4876
ISSN (Print):   2042-4868
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