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2013 (Vol. 5, Issue: 11)
Research Article

Physico Chemical Properties and Antioxidant Activity of Roselle Seed Extracts

Abdoulaye Idrissa Cissouma, Fatoumata Tounkara, Mehdi Nikoo, Na Yang and Xueming Xu
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122 Jiangsu, P.R. China

DOI: 10.19026/ajfst.5.3371
Submitted Accepted Published
July 17, 2013 July 31, 2013 November 05, 2013

  How to Cite this Article:

Abdoulaye Idrissa Cissouma, Fatoumata Tounkara, Mehdi Nikoo, Na Yang and Xueming Xu, 2013. Physico Chemical Properties and Antioxidant Activity of Roselle Seed Extracts.  Advance Journal of Food Science and Technology, 5(11): 1483-1489.

DOI: 10.19026/ajfst.5.3371

URL: http://www.maxwellsci.com/jp/mspabstract.php?jid=AJFST&doi=ajfst.5.3371


The aim of this research was to extract phenolic compounds from defatted Roselle (Hibiscus sabdariffa L.) seed and assess their antioxidant potential. Water, ethanol (30%), methanol (30%) and acetone (30%) were used as solvent for extraction. The proximate composition, total phenolic content and extraction yield were analyzed. Antioxidant efficacies of Roselle seed extract were tested by using 1, 1-Diphenyl-2-Picrylhydrazyl (DPPH), hydroxyl, 2, 2-azinobis-3-ethylbenzothiaz oline-6-sulfonic acid (ABTS) radicals scavenging capacities and reducing power analysis. Roselle seeds were found to be rich in protein (27.745%), carbohydrates (40.45%) and oil (20.83%). The total phenolic content ranged from 1.660.03 to 1.990.01 (GAE) mg/g using water and 30% acetone respectively. The highest inhibitory capacity on DPPH and ABTS radicals was observed in 30% acetone extract and was at 3 mg/mL for DPPH and 6 mg/mL for ABTS respectively. Ethanol extract showed the highest hydroxyl radical scavenging ability value of 66.36 at 20 mg/mL, followed by methanol (57.27), acetone (56.36) and water (30). The reducing potential of the different extracts was concentration dependent and increased with increase in concentration. These results indicate that substantial antioxidant activity can be obtained from Roselle seed phenolic compounds extract by using 30% acetone.

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    Competing interests

The authors have no competing interests.
    Open Access Policy

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.


© The Author(s) 2013

ISSN (Online):  2042-4876
ISSN (Print):   2042-4868
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