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2017 (Vol. 8, Issue: 1)
Research Article

Physical Charaterization of Chromatographic Components from the Bioactive Aqueous Fraction of Vernonia amygdalina [Esteraeceae] Leaf

1P.C. Adiukwu, 2P. Waako, 3G. Nambatya and 1T.C. Fleischer
1School of Pharmacy, University of Hsealth and Allied Sciences, P.M.B. 31 Ho, Ghana
2Faculty of Health Sciences, Busitema University, P.O. Box 236 Busitema, Eastern Region
3Natural Chemotherapeutic Research Laboratories, P.O. Box 4864 Kampala, Uganda

DOI: 10.19026/bjpt.8.5127
Submitted Accepted Published
April 12, 2017 June 23, 2017 July 25, 2017

  How to Cite this Article:

1P.C. Adiukwu, 2P. Waako, 3G. Nambatya and 1T.C. Fleischer, 2017. Physical Charaterization of Chromatographic Components from the Bioactive Aqueous Fraction of Vernonia amygdalina [Esteraeceae] Leaf.  British Journal of Pharmacology and Toxicology, 8(1): 1-8.

DOI: 10.19026/bjpt.8.5127

URL: http://www.maxwellsci.com/jp/mspabstract.php?jid=BJPT&doi=bjpt.8.5127


The objective of this study was to isolate and characterize the bioactive saponin from Vernonia amygdalina leaf using its physical and chromatographic properties. Standard procedure of liquid-liquid extraction was employed to isolate crude saponin and column chromatography to obtain fractionate for bio-evaluation. Antipyretic evaluation of the crude saponin and fractionate was carried out using Saccharomyces cerevisiae induced pyrexia. Pure components from fractionate were separated using Reverse-Phase High Performance Liquid Chromatography. The physical properties of these components were evaluated according to standard procedures using organoleptic, ignition and solubility tests. Obtained data indicated three pure colored components with retention times 7.780.19, 8.760.16 and 13.540.87 min. Data obtained showed all the components to be aromatic and polar in character. Finding suggests that the bioactive saponin is triterpenoidal.

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    Competing interests

The authors have no competing interests.
    Open Access Policy

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.


© The Author(s) 2017

ISSN (Online):  2044-2467
ISSN (Print):   2044-2459
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