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2016 (Vol. 8, Issue: 4)
Research Article

Molecular Detection and Viral Isolation of Newcastle Disease Viruses from Chickens in Benue State, Nigeria

1H.O. Abah, 2P.A. Abdu and 3A. Assam
1Department of Veterinary Medicine, College of Veterinary Medicine, University of Agriculture Makurdi, Benue State
2Department of Veterinary Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University Zaria
3Depertment of Animal Science, Faculty of Agriculture and Forestry, Cross River University of Technology, Obubura, Cross River State, Nigeria

DOI: 10.19026/ijava.8.3340
Submitted Accepted Published
May ‎13, ‎2016 June ‎25, ‎2016 November 20, 2016

  How to Cite this Article:

1H.O. Abah, 2P.A. Abdu and 3A. Assam, 2016. Molecular Detection and Viral Isolation of Newcastle Disease Viruses from Chickens in Benue State, Nigeria.  International Journal of Animal and Veterinary Advances, 8(4): 40-45.

DOI: 10.19026/ijava.8.3340

URL: http://www.maxwellsci.com/jp/mspabstract.php?jid=IJAVA&doi=ijava.8.3340


The present research work was carried out to detect and isolate Newcastle Disease Virus (NDV) from apparently healthy chickens and evaluate their role in Newcastle disease outbreak.A survey on the prevalence of Newcastle Disease Virus (NDV) was conducted in six Local Government Areas (LGAs) of Benue State, Nigeria. A total of 250 cloacal and tracheal swabs were collected from apparently healthy chickens from commercial farms, Live Bird Markets (LBMs) and village households. Reverse Transcription-Polymerase Chain Reaction (RT-PCR) was used to detect Newcastle Disease Viruses (NDV) while viral isolation was performed using embryonated chicken eggs. The overall detection rate for ND Viruses (NDVs) was 12% (30/250). Newcastle disease viruses were detected in all sampling units with the highest viral detection of 16.1% (9/56) from chickens in LBMs. The overall NDV isolation rate was 20% (6/30), isolates were from Kwande and Makurdi LGAs with village chickens from LBMs (55.6%) recording the highest isolation rate. Chickens from LBMs, Kwande and Makurdi LGAs may serve as sources of NDVs to those in commercial farms and village households. The vaccination of village chickens should be considered as important in the control of ND in Benue State, Nigeria.

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    Competing interests

The authors have no competing interests.
    Open Access Policy

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.


© The Author(s) 2016

ISSN (Online):  2041-2908
ISSN (Print):   2041-2894
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