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Abstract
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Article Information:
Concentration-Dependent Effect of Ivermectin on Adenosine Triphosphatase (Atpase) Activities in Human Erythrocytes
Miriam O. Aniagolu, Elvis N. Shu, Joel O. Onyeanusi, Emeka E. Neboh and Ebele J. Ikekpeazu
Corresponding Author: E. Neboh Emeka
Submitted: December 20, 2011
Accepted: January 12, 2012
Published: April 30, 2012 |
Abstract:
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Ivermectin remains the drug of choice for Onchocerciasis treatment and previous studies have
documented an inhibition of Adenosine Triphosphatase (ATPases) activities in adult Onchocerca volvulus. The
study is aimed at examining the effects of Ivermectin on ATPase activities of human erythrocytes. Sixty (60)
subjects, (30 males and 30 females) residing in Enugu metropolis, were recruited for the study. Venous blood
samples (3 mL) were collected from each subject from where erythrocyte ghosts were prepared. Erythrocyte
ghosts were prepared via hypotonic lysis, using ice-cold sodium phosphate at pH 7.4. Total ATPase,
magnesium (Mg2+) and Calcuim (Ca2+) ATPase activities were assayed using standard spectrophotometric
methods, before and after Ivermectin administration on the erythrocyte ghosts, whereas Na+/K+ ATPase activity
was calculated. Data analysis was done by one-way analysis of variance (ANOVA), Turkey’s multiple
comparison test and Student’s t-test. After treatment, the mean Mg2+ ATPase activity showed a significant 3-
fold increase (p<0.0001; F-ratio = 11.765) compared to the mean basal activity. There was no significant effect
on Na+/K+ ATPase and Ca2+ ATPase activities (p>0.05; F-ratio = 0.6480), (p>0.05; F-ratio = 0.9151),
respectively. There was non-significant difference between the mean basal activities of the different ATPases
in the different sexes, but Mg2+ ATPase activity of the males was significantly higher (p<0.05) than females
after administration of 20-40 ng/mL of Ivermectin, whereas Na+/K+ ATPase activity was higher in males after
the addition of 40-50 ng/mL of the drug. The significant activation of Mg2+ ATPase observed might increase
its function of energy generation for maintenance of the biconcave shape of erythrocytes. Ivermectin
administration affected the activities of Mg2+ and Na+/K+ ATPases. There is no plausible reason for the observed
sex difference.
Key words: Effects, erythrocyte ghosts, Ivermectin, onchocerciasis, trans-membrane, ,
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Cite this Reference:
Miriam O. Aniagolu, Elvis N. Shu, Joel O. Onyeanusi, Emeka E. Neboh and Ebele J. Ikekpeazu, . Concentration-Dependent Effect of Ivermectin on Adenosine Triphosphatase (Atpase) Activities in Human Erythrocytes. Asian Journal of Medical Sciences, (2): 61-65.
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ISSN (Online): 2040-8773
ISSN (Print): 2040-8765 |
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