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Abstract
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Article Information:
Stress Response in Infective Larvae (L3) of the Parasitic Nematode Haemonchus contortus is Accompanied by Enhanced Expression of Heat Shock Proteins (HSP 70)
D.N. Siamba, P.N. Gatongi, C.S. Mulambalah, M.M. Ngeiywa and L.W. Wamae
Corresponding Author: Siamba
Submitted: April 04, 2011
Accepted: May 13, 2011
Published: July 10, 2012 |
Abstract:
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The study was undertaken to investigate the effects of temperature and moisture stresses on protein
profile and characteristics in the free living third larval stages (L3) of Haemonchus contortus. Before and after
temperature and moisture stress treatment, the L3 were tested for the induction and expression of Heat-Shock
Proteins (HSPs). Proteins were analyzed by Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis
(SDS-PAGE) and immunoblot analysis with alkaline-phosphatase conjugated anti HSP 70 antibody. The stress
resulted in an altered protein pattern. It was also revealed that stress treatment did not alter the signals obtained
with the anti HSP 70 antibody, but the amount of HSP70 as estimated by densitometry differed between pre
and post stress samples. This response in the free living third stage larvae (L3) of H. contortus may be aimed
at protecting the parasite against molecular damage and ensuring survival during stressful periods.
Key words: Haemonchus, heat shock proteins, moisture, stress, temperature, ,
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Abstract
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Cite this Reference:
D.N. Siamba, P.N. Gatongi, C.S. Mulambalah, M.M. Ngeiywa and L.W. Wamae, . Stress Response in Infective Larvae (L3) of the Parasitic Nematode Haemonchus contortus is Accompanied by Enhanced Expression of Heat Shock Proteins (HSP 70). Current Research Journal of Biological Sciences, (4): 345-349.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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