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Abstract
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Article Information:
Identification of Rapd Primers Linked to Leaf Rust Resistant Gene Lr51 Introgressed from Triticum speltoides to Common Wheat (Triticum aestivum L.)
Khaist Begum, Habib Ahmad, Sajid ul Ghafoor, Sahib Gul Afridi, Ikram Muhammad and Imtiaz Ahmed Khan
Corresponding Author: Imtiaz Ahmed Khan
Submitted: 2009 December, 29
Accepted: 2010 January, 23
Published: 2010 July, 20 |
Abstract:
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Leaf rust is the most important disease of wheat all over the world. Geneticists and breeder are now
extracting new leaf rust resistant genes from wild relatives of wheat. An important leaf rust resistant gene Lr51
has been transferred from Triticum speltoides to common wheat. Present research was carried out to develop
Randomly Amplified Polymorphic DNA (RAPD) based marker for the leaf rust resistant gene Lr51. Out of
seven RAPD primers used, one RAPD prime viz; GLA-09 was found which could be used reliably to keep track
of Lr51 in wheat background. This primer (GLA-09) amplified a polymorphic DNA fragment of approximately
1100 bp in the wheat lines having the leaf rust resistant gene Lr51.
Key words: Leaf rust, Lr51, molecular marker, PCR, RAPD, Triticum aestivum L., Triticum speltoides
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Cite this Reference:
Khaist Begum, Habib Ahmad, Sajid ul Ghafoor, Sahib Gul Afridi, Ikram Muhammad and Imtiaz Ahmed Khan, . Identification of Rapd Primers Linked to Leaf Rust Resistant Gene Lr51 Introgressed from Triticum speltoides to Common Wheat (Triticum aestivum L.). Current Research Journal of Biological Sciences, (4): Page No: 226-228.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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