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Article Information:
DNA Based Characterization of Various Morel Species
Naheed Fazal, Habib Ahmad, Zubeda Chaudhry, Shaista Bibi, Zafar Ali Yousafzai and Imtiaz Ahmed Khan
Corresponding Author: Imtiaz Ahmad Khan
Submitted: 2011 March, 16
Accepted: 2011 August, 27
Published: 2012 January, 25 |
Abstract:
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Morels (Morchella species) are important edible mushrooms belonging to family Helveliaceae.
Despite it’s economic and medicinal importance, not much scientific work (especially molecular work) has
been done in Pakistan for improvement of morels. During present research, genomic DNA profiles of five
morels species obtained from upper Swat valley, Khyber Puknhtoonkhawa province were studied using four
Randomly Amplified Polymorphic (RAPD) primers GLE-05, -06, -07 and -09. Moderate amount of genetic
diversity (average GD ranging from 27-62%) was observed among the material studied. The DNA fragments
amplified using 4 RAPD primers ranged in size from approximately 100 to 1100 bp. Two, 3.0, 4.6 and 2.2
alleles per accession were amplified using GLE-05, -06, -07 and -09, RAPD primers, respectively. To elaborate
phylogenetic relationship among the accessions, a dendrogram was constructed. It is recommended that similar
kinds of research should be conducted on larger scale so that a more clear estimate of existing genetic diversity
in Pakistani morels can be established which will help in formulating better breeding programs for the
improvement of morels in Pakistan.
Key words: Genetic distance, morels, Morchella species, PCR, phylogeny, RAPD,
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Cite this Reference:
Naheed Fazal, Habib Ahmad, Zubeda Chaudhry, Shaista Bibi, Zafar Ali Yousafzai and Imtiaz Ahmed Khan, . DNA Based Characterization of Various Morel Species. Asian Journal of Agricultural Sciences, (1): 5-7.
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ISSN (Online): 2041-3890
ISSN (Print): 2041-3882 |
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