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Abstract
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Article Information:
In vitro Propagation of Citrus limonia Osbeck Through Nucellar Embryo Culture
Alka Jajoo
Corresponding Author: Alka Jajoo
Submitted: 2009 July, 11
Accepted: 2009 July, 27
Published: 2010 January, 05 |
Abstract:
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Citrus limonia Osbeck is a promising rootsctock for commercial citrus species with sturdy and
disease and drought resistant characters. A n efficient and highly reproducible plant regeneration protocol has
been developed from nucellar embryo of Citrus limonia. Murashige and Skoogs medium was used for plant
regeneration from nucellar embryos. It was noted that 6-benzylaminopurine at a concentration of 2.22 mM
induced highest number of multiple shoots as 18.26 shoots per explant. On transfer of individual shoots to root
inducing MS medium supplied with auxins, Indole-3-butyric acid (2.46 µM) and 6-benzylaminopurine
(1.11 µM) proved to be the best combination. This media resulted in 78.80 % rooting and produced plantlets
with an average of 5.53 roots/ shoot. Plantlets thus obtained were successfully transferred to green house for
further observations. The micropropagated plantlets thus obtained can be used as rootstocks by traditional
method or by micrografting.
Key words: Citrus limonia, Micrografting, Propagation, Resistant, Rootstock, ,
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Abstract
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Cite this Reference:
Alka Jajoo, . In vitro Propagation of Citrus limonia Osbeck Through Nucellar Embryo Culture. Current Research Journal of Biological Sciences, (1): Page No: 6-8.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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