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Abstract
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Article Information:
Novel Polymorphic Microsatellites from Guppy (Poecilia reticulata) and their Utility in Swordtails (Xiphophorus helleri)
Gen Hua Yue and Laszlo Orban
Corresponding Author: G.H. YUE
Submitted: 2010 January, 29
Accepted: 2010 February, 11
Published: 2010 May, 20 |
Abstract:
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Ten microsatellites were isolated from a genomic DNA library generated from guppy (Poecilia
reticulata; Poecilidae) enriched for C A-repeats. All of the 10 m icrosatellites were polymorphic in guppy with
an average allele number of 4.9/locus ranging from 2 to 14. All 10-prim er pairs amplified specific products in
green swordtail (Xiphophorus helleri) and 9 of the 10 microsatellites displayed polymorphism (average allele
number: 4.1/locus with a scope between 2 and 8). Size range of alleles at most loci were similar between the
two fish species. These microsatellites could be applied to breeding programs performed on these two species
and possibly other poecilids and to genetic and ecological studies.
Key words: Cross-amplification, directional mutation, guppy, swordtail, , ,
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Cite this Reference:
Gen Hua Yue and Laszlo Orban, . Novel Polymorphic Microsatellites from Guppy (Poecilia reticulata) and their Utility in Swordtails (Xiphophorus helleri). Current Research Journal of Biological Sciences, (3): Page No: 161-163.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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