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Abstract
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Article Information:
Estimation of Genetic Diversity in Sheep (Ovis aries) using Randomly Amplified Polymorphic DNA
M. Qasim, H. Ahmad, S. Ghafoor, S.G. Afridi, I. Muhammad and A.K. Imtiaz
Corresponding Author: Imtiaz Ahmed Khan
Submitted: 2010 August, 07
Accepted: 2010 September, 01
Published: 2011 February, 05 |
Abstract:
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Domestic sheep (Ovis aries) belongs to order Artiod actyla. Despite its great economic importance,
not much work has been done in Pakistan on breeding and genetics of sheep. Present research was initiated to
analyze DNA based genetic diversity in Pakistani breeds of sheep. Total genomic DNA was isolated from
blood of Rambouillet, Kaghani and Ramghani breeds. Polymerase Chain Reactions (PCR) were carried out
using six Randomly Amplified Polymorphic DNA (RAPD) primers. Amplified fragments ranged in size from
500 bp to 2000 bp. On average, 3 alleles per genotype were amplified. Average genetic distance estimate
ranged from 30-100%. Eleven comparisons showed maximum genetic diversity (GD = 100%) while 6
comparisons sowed minimum genetic diversity (GD = 30%) for the loci detected using 6 RAPD primers.
Phylogenetic elaboration among the 14 sheep accessions were carried out using dendrogram analysis. The 14
accessions of sheep were grouped into 5 clusters comprising 3 , 3, 3, 3 and 2 accessions, respectively.
Key words: Dendrogram, genetic diversity, Ovis aries, phylogeny, RAPD, sheep,
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Cite this Reference:
M. Qasim, H. Ahmad, S. Ghafoor, S.G. Afridi, I. Muhammad and A.K. Imtiaz, . Estimation of Genetic Diversity in Sheep (Ovis aries) using Randomly Amplified Polymorphic DNA. International Journal of Animal and Veterinary Advances, (1): 6-9.
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ISSN (Online): 2041-2908
ISSN (Print): 2041-2894 |
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