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Abstract
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Article Information:
Technology Optimization of Enzymolysis of Burmuda Grass
Liu Jun-Hong, Yang Ru, Wang Fu-Mei, Zhao Qi and Cheng Hui
Corresponding Author: Wang Fu-mei
Submitted: December 26, 2012
Accepted: January 25, 2013
Published: March 29, 2014 |
Abstract:
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Cellulose can be degraded by enzyme to glucose, which provide carbon source for ethanol fermentation. This study, taking Burmuda grass as material, analyzed effects of temperature, time, pH, PBS dosage and ratio of enzyme on reducing sugar. It came to the conclusion that the best conditions of the enzymolysis of Burmuda grass are as follows: 50°C for temperature, 32 h for time, 4.20 for pH, 2 mL for PBS dosage, 4:3 for the ratio of xylanase and cellulase, the yield of reducing sugar reached 78.63%.
Key words: Bioethanol, cellulose, orthogonal experiment, reducing sugar, standard curve, xylanase and cellulase,
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Cite this Reference:
Liu Jun-Hong, Yang Ru, Wang Fu-Mei, Zhao Qi and Cheng Hui, . Technology Optimization of Enzymolysis of Burmuda Grass. Research Journal of Applied Sciences, Engineering and Technology, (12): 2411-2415.
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ISSN (Online): 2040-7467
ISSN (Print): 2040-7459 |
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