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Abstract
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Article Information:
Identification of RAPD Marker for Chromosome 1D of Common Wheat
Shaista Bibi, Habib Ahmad, Khaist Begum, Sajid ul Ghafoor and Imtiaz Ahmad Khan
Corresponding Author: Imtiaz Ahmed Khan
Submitted: 2009 December, 22
Accepted: 2010 January, 11
Published: 2010 April, 10 |
Abstract:
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Development of genetically compensating nullisomic-tetrasomic and ditelosomic lines of common
wheat (Triticum aestivum L.) have been widely used to construct high density genetic maps of homoeologous
wheat chromosomes. During present research, easier, cheaper and quicker procedure of Polymerase Chain
Reaction (PCR) was used to map Randomly Amplified Polymorphic DNA primers on chromosome 1D of
common wheat. Genomic DNA was isolated from two genetic stocks of wheat cultivar Chinese Spring viz;
NT-1D1B and NT-2A2B. PCR were conducted using RAPD primers GLC-07 and GLC-11. RAPD primer
GLC-11 amplified a polymorphic allele of approximately 500 bp, which was present in NT-2A2B (used as
positive control) but was absent in NT-1D1B indicating that the locus is present on chromosome 1D of common
wheat. Hence this marker (GLC-11) can reliably be used to keep track of chromosome 1D of hexaploid wheat.
Key words: Biotechnology, chromosome mapping, DNA, molecular genetics, molecular marker, nullisomic tetrasomic lines, PCR, randomly amplified polymorphic DNA, triticum aestivum, wheat genetics
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Cite this Reference:
Shaista Bibi, Habib Ahmad, Khaist Begum, Sajid ul Ghafoor and Imtiaz Ahmad Khan, . Identification of RAPD Marker for Chromosome 1D of Common Wheat. Asian Journal of Agricultural Sciences, (2): Page No: 51-52.
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ISSN (Online): 2041-3890
ISSN (Print): 2041-3882 |
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