Abstract
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Article Information:
Sequence Analysis of α-gliadin Genes from Aegilops tauschii Native to China
Guangrong Li, Tao Zhang, Pei Wei, Juqing Jia and Zujun Yang
Corresponding Author: Zujun Yang
Submitted: 2010 June, 07
Accepted: 2010 July, 03
Published: 2010 October, 09 |
Abstract:
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Aegilops tauschii was a D-genome progenitor for cultivated wheat (Triticum aestivum L). The
accessions of Ae. tauschii native to China contained novel agronomically important traits including unique seed
storage proteins for modern wheat improvement. Total 19 α-gliadin gene sequences were isolated from Ae.
tauschii accession from Henan province. Five of 19 sequences contained in-frame stop condons and were
predicted to be pseudogenes, suggesting the high variation of gliadin genes in Ae. tauschii genome. The Open
Reading Frame (ORF) lengths of these sequences encoded 281-303 residues, with the repetitive polyglutamine
from 17-28 residues. There are two α-gliadin sequences present either 5 or 7 cysteine residues, which possibly
related to high quality. Four peptides of T cell stimulatory epitopes in Celiac Disease (CD ) patients were
distributed in most Ae. tauschii α-gliadin gene sequences. In comparison with the reported α-gliadin sequences
in wheat ancestral species, the sequences of Ae. tauschii displayed high haplotypes diversity and significant
deviation from a neutral distribution, indicative of the fast evolution of α-gliadin genes in Ae. tauschii species.
Key words: Aegilops tauschii, α-gliadin, evolutionary analysis, wheat breeding, , ,
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Cite this Reference:
Guangrong Li, Tao Zhang, Pei Wei, Juqing Jia and Zujun Yang, . Sequence Analysis of α-gliadin Genes from Aegilops tauschii Native to China. Asian Journal of Agricultural Sciences, (4): Page No: 128-135.
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ISSN (Online): 2041-3890
ISSN (Print): 2041-3882 |
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