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Abstract
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Article Information:
The Effect of Hypoxia on G Protein Coupled (Opioid) Receptor Gene Expression in Cortical B50 Neurons in Culture
A.O. Ibegbu, I. Mullaney, L. Fyfe and D. McBean
Corresponding Author: Augustine Ibegbu
Submitted: 2011 January, 26
Accepted: 2011 February, 23
Published: 2011 April, 30 |
Abstract:
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Hypoxia adversely affects cells and tissues, and neuronal cells in particular have been shown to be
more susceptible to the injurious effects of hypoxia in which they may begin to die when oxygen supply is
reduced or completely eliminated. Opioid receptor agonists have been shown to elicit several central nervous
system effects, mediated via G protein-coupled receptors. The aim of this study was to study the effect of
hypoxia on G protein coupled receptor gene expression using mu opioid receptor as a case study in cortical
neuronal B50 cell lines in culture. The B50 cells were cultured in normoxia (21% O2; 5% CO2) and hypoxia
(5% O2; 5% CO2), and were treated with opioid agonists to determine their effects on hypoxia-induced changes.
Three opioid agonists {DAMGO(μ), DSLET(δ) and ICI--199,441(κ)}, were administered to the cells as
treatment for 48 h after 48 h of initial culture for a total of 96 h of culture in hypoxic conditions
at concentrations of 10, 50 and 100 μM. The levels of G-protein coupled receptor (mu opioid) mRNAs were
assessed using RT-PCR. The results showed that hypoxia induced morphological changes in B50 cells in
hypoxia while the mu opioid RT-PCR mRNA levels showed no appreciable changes in normal, hypoxic and
treated cells. The results show that B50 neuronal cells are susceptible to damage and injurious effects of
hypoxia, as are most brain cells and the opioid agonist treatments showed there were no changes in the level
of mu opioid receptor gene expression due to hypoxia or agonist treatment in neuronal B50 cells in culture.
Key words: G-protein coupled receptor, hypoxia, messenger RNA, opioid agonist, opioid (mu) receptor, reverse transcription-PCR,
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Abstract
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Cite this Reference:
A.O. Ibegbu, I. Mullaney, L. Fyfe and D. McBean, . The Effect of Hypoxia on G Protein Coupled (Opioid) Receptor Gene Expression in Cortical B50 Neurons in Culture. British Journal of Pharmacology and Toxicology, (2): 75-83.
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ISSN (Online): 2044-2467
ISSN (Print): 2044-2459 |
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