|
Abstract
|
Article Information:
Isolation and Partial Characterization of Two Plant Lectins
S.N. Laija, S. Mahesh, L.S. Smitha and P. Remani
Corresponding Author: Laija S Nair
Submitted: 2010 March, 30
Accepted: 2010 April, 13
Published: 2010 July, 20 |
Abstract:
|
Plant lectins with different sugar specificities were isolated from the seeds of Artocarpus integrifolia
and Canavalia gladiata using a procedure that involved affinity chromatography. Of the two lectins isolated,
protein content was found to be more in Artocarpus integrifolia than in Canavalia gladiata whereas
carbohydrate content was more in Canavalia gladiata. Both the lectins were found to be stable up to 100ºC.
Lectin from Artocarpus integrifolia showed two bands with molecular weight 11 and 17 kDa. Canavalia gladiata also gave two dissimilar bands of molecular weight 26 and 17 kDa both in the presence and absence
of $-mercaptoethanol. Carbohydrate staining of SDS gel indicated that both the bands are glycosylated
Canavalia gladiata whereas only one band was glycosylated in Artocarpus integrifolia.
Key words: Artocarpus integrifolia, Canavalia gladiata, lectin, mannose, N-acetyl galactosamine, ,
|
|
Abstract
|
PDF
|
HTML |
|
Cite this Reference:
S.N. Laija, S. Mahesh, L.S. Smitha and P. Remani, . Isolation and Partial Characterization of Two Plant Lectins. Current Research Journal of Biological Sciences, (4): Page No: 232-237.
|
|
|
|
 |
ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
 |
| Information |
|
|
|
|
| Sales & Services |
|
|
|
|
