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Abstract
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Article Information:
Response Surface Methodology for the Optimization of Lactoferrin Nano-Liposomes
Jieqing Ma, Rongfa Guan, Chang Ri, Mingqi Liu, Xingqian Ye and Jiaxin Jiang
Corresponding Author: Rongfa Guan
Submitted: July 17, 2012
Accepted: August 28, 2012
Published: October 20, 2012 |
Abstract:
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The purpose of this study was to optimize the formulation of lactoferrin nano-liposomes using response
surface methodology. Response surface methodology based on central composite rotatable design has been
successfully used to model and optimize biochemical and biotechnological processes. The mass ratio of
phosphatidylcholine and cholesterol (1-8), lactoferrin concentration (2-15 mg/mL), tween80 (0.5-2.5 mg/mL) and
the volume ratio of organic phase and aqueous phase (2-10) were selected as independent variables with
encapsulation efficiency and particle size as dependent variables. For each response, a 2nd-order polynomial model
was developed using multiple linear regression analysis. Applying a desirability function method the optimum
parameters were: phosphatidylcholine to cholesterol mass ratio of 5.77, lactoferrin concentration of 11.71 mg/mL,
tween80 of 1.18 mg/mL and organic phase to aqueous phase volume ratio of 4.59. At this optimum point, particle
size and encapsulation efficiency were found to be 214 nm and 60.21%, respectively. Furthermore, leakage ratio of
nano-liposomes was used to determine the influence of storage period.
Key words: Encapsulation efficiency, lactoferrin, nano-liposomes, particle size, response surface methodology, stability,
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Cite this Reference:
Jieqing Ma, Rongfa Guan, Chang Ri, Mingqi Liu, Xingqian Ye and Jiaxin Jiang, . Response Surface Methodology for the Optimization of Lactoferrin Nano-Liposomes. Advance Journal of Food Science and Technology, (5): 249-256.
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ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
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