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Abstract
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Article Information:
Lipase Production by Bacillus subtilis OCR-4 in Solid State Fermentation Using Ground Nut Oil Cakes as Substrate
Manoj Singh, Kumar Saurav, Neha Srivastava and Krishnan Kannabiran
Corresponding Author: Manoj Singh
Submitted: 2010 April, 30
Accepted: 2010 May, 17
Published: 2010 July, 20 |
Abstract:
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A moderately alkallophillic strain of Bacillus subtilis was isolated and comparative study on the
production of extra cellular lipase by Solid State Fermentation (SSF) with various substrates has been evaluated.
Different intends such as temperature, pH, different substrates and effect of incubation time of the medium were
optimized for maximum yield. The maximum extracellular lipase activity of 4.5 units per gram of dry fermented
substrates (Ug/ds) was observed with ground nut oil cake after 48 h of fermentation with 70% initial moisture
content of the substrate and suitable growth of bacterial mass culture was for maximum yield of lipase at pH
8 was observed. Solid-state fermentation is a high recovery method for the production of industrial enzymes.
Key words: Lipase, Bacillus subtilis, Solid State Fermentation (SSF), substrates, , ,
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Cite this Reference:
Manoj Singh, Kumar Saurav, Neha Srivastava and Krishnan Kannabiran, . Lipase Production by Bacillus subtilis OCR-4 in Solid State Fermentation Using Ground Nut Oil Cakes as Substrate. Current Research Journal of Biological Sciences, (4): Page No: 241-245.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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