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Abstract
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Article Information:
Genetic Relationships of two Sonchus Species Collected from two Locations in Khartoum State Using RAPD Markers
H.H. Elkamali, M.S.M. El-Kheir, R.S. Habeballa, N.B. Hamaza, I.E. Abdalla, E.I. Ahmedani, A.A.S. Mohammed, B.A.A. Abuzaid, M.B. Mohammed and T.Y. Ahmed
Corresponding Author: Hatil Hashim EL-Kamali
Submitted: 2010 October, 11
Accepted: 2010 November, 15
Published: 2011 March, 05 |
Abstract:
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Random amplified polymorphic DNA markers were used to assess genetic diversity in Sonchus
oleraceus and Sonchus cornatus (Asteraceae), vulnerable medicinal plants collected from two locations
(Shambat and Omdurman) in Khartoum state. The DNA extracted by Cetyltrimethyl ammonim bromide method
(CTAB) from the plant sample was amplified by successive PCR cycles. Using 15 random primers, the
amplified DNA was subjected to agarose gel electrophoresis. 74 different bands were observed under UV light.
A total of 51 polymorphisms (3.4 polymorphic markers per primer) out of 74 (4.9 fragments per primer) were
obtained from the 15 primers used. Unweighted Pair Group Method with Arithmetic Averages (UPGMA)
clustering resulted in two major groups. According to the dendrogram, two groups were obtained based on
genetic distance. The first group is indicated by A1 and A2 which were genetically similar. The second group
included B1 and B2. S. oleraceus and S. cornatus from Shambat area clustered together on dendrogram and
those from Omdurman area in other cluster.
Key words: RAPD fingerprinting, genetic diversity, Sonchus spp., Sudan, , ,
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Cite this Reference:
H.H. Elkamali, M.S.M. El-Kheir, R.S. Habeballa, N.B. Hamaza, I.E. Abdalla, E.I. Ahmedani, A.A.S. Mohammed, B.A.A. Abuzaid, M.B. Mohammed and T.Y. Ahmed, . Genetic Relationships of two Sonchus Species Collected from two Locations in Khartoum State Using RAPD Markers. Current Research Journal of Biological Sciences, (2): 95-99.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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