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Article Information:
Correlation of Chemiluminescence and Electrochemiluminescence Methods for Determination of Serum Free Triiodothyronine Level in Cow
Behrad Eshratkhah, Mehrdad Mostafaei Bigham, Alireza Mirzaei and Rahim Beheshti
Corresponding Author: Behrad Eshratkhah
Submitted: 2011 May, 21
Accepted: 2011 June, 28
Published: 2011 August, 30 |
Abstract:
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The aim of this study was to determine serum free-triiodothyronine (fT3) level and its correlation
between the CLIA and ECLIA methods in cow. Blood samples were collected from the jugular vein of 25
clinically healthy animals aged from 2 to 4 years. This study was performed in August. The separated sera were
analyzed to measurement of fT3 concentration using the CLIA and ECLIA methods. Our results indicate that
there was no a significant difference between two method concerning the fT3 concentration in cow, but its mean
was higher in CLIA method. The regression analysis revealed a significant correlation between two methods:
(p = 0.022, r = 0.460 for fT3). It was concluded that the CLIA and ECLIA methods can be used as an alternative
to the previous methods for assaying the fT3 concentration in veterinary diagnostic laboratories and exhibited
an acceptable of sensivity and precision for the serum fT3 determination in cow.
Key words: Chemiluminescence immunoassay, cow, electrochemiluminescence immunoassay, freetriiodothyronine, serum, ,
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Abstract
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Cite this Reference:
Behrad Eshratkhah, Mehrdad Mostafaei Bigham, Alireza Mirzaei and Rahim Beheshti, . Correlation of Chemiluminescence and Electrochemiluminescence Methods for Determination of Serum Free Triiodothyronine Level in Cow. International Journal of Animal and Veterinary Advances, (4): 245-248.
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ISSN (Online): 2041-2908
ISSN (Print): 2041-2894 |
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