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Abstract
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Article Information:
The Effects of Crude Methanol Seed Extract of Brassica juncea on Haematological Parameters in Wistar Rats
S.A. Ufelle, E.O. Ukaejiofo, E.E. Neboh, P.U. Achukwu, S. Ghasi, J.E. Ikekpeazu and I.C. Maduka
Corresponding Author: Silas Ufelle
Submitted: 2011 March, 25
Accepted: 2011 May, 10
Published: 2011 August, 05 |
Abstract:
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Brassica juncea, (B. juncea) also known as Indian Oriental or Brown Mustard is widely used as a
medicinal crop, oil seed, condiment and vegetable and much hematological effects have not been recorded on
B. juncea. Thirty (30) male wistar rats aged 2-3 months, weighing 100-200 g were obtained and housed at the
Animal House of College of Medicine, University of Nigeria Enugu Campus for this study. The rats were
divided into five (5) groups of six (6) rats per group, labeled A to E and were acclimatized for two (2) weeks
and were fed with commercially available rat feed. They had access to water and feed ad libitum. Groups A
to D were orally administered with graded doses of the crude seed extract (50, 100, 200 and 300 mg/kg body
weight respectively) daily for 30 days while group E which served as control received no extract. Blood
samples (2.0 mL) were collected from each rat through the retro orbital plexus of the median canthus into K3-
EDTA containers for analysis using standard operative procedures. The results obtained, revealed that the mean
Hemoglobin (Hb) values of groups C and D (C = 12.6±0.57 and D = 11.1±1.4 g/dL) were significantly
decreased (p<0.05) when compared with control (E = 14.2±0.74 g/dL. The mean Hematocrit (PCV) values of
groups C and D (C = 0.38±0.02, and D = 0.34±0.04 L/L) were also significantly decreased (p<0.05) compared
with control (E = 0.43±0.02 L/L). The mean Total WBC values of all the treated groups were significantly
increased (A = 6.45±0.3, B = 6.23±0.83, C = 6.0±0.34, and D = 6.05±0.41 x 109/L) when compared with
control E = 4.28±0.17×109/L (p<0.05). The mean platelet values were significantly decreased in all the treated
groups (A = 30.00±4.1, B = 30.00±5.8, C = 46.25±7.5 and D = 33.75±2.5 x 109/L) when compared with control
E = 86.25±4.8×109/L. This study revealed that crude methanol seed extract of Brassica juncea affects some
hematological parameters when orally administered at different concentrations.
Key words: Animal models, Brassica juncea, graded dosing, hematocrit, treated groups, ,
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Cite this Reference:
S.A. Ufelle, E.O. Ukaejiofo, E.E. Neboh, P.U. Achukwu, S. Ghasi, J.E. Ikekpeazu and I.C. Maduka, . The Effects of Crude Methanol Seed Extract of Brassica juncea on Haematological Parameters in Wistar Rats. British Journal of Pharmacology and Toxicology, (3): 123-126.
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ISSN (Online): 2044-2467
ISSN (Print): 2044-2459 |
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