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Abstract
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Article Information:
Biochemical Characterization of Rice Somaclones Resistant to Blast
Antar N. El-Banna and Ismael A. Khatab
Corresponding Author: A.N. El-Banna
Submitted: 2011 October, 17
Accepted: 2011 November, 25
Published: 2012 March, 10 |
Abstract:
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The aim of the present study was to produce rice somaclones resistant to P. oryza that could be useful
to improve rice plants against blast disease and use them in the future breeding programs. In addition, to detect
biochemical changes among somaclones and their original cultivars. Mature embryo-derived calli of the three
susceptible rice varieties, Sakha 101, Sakha 104 and Riho were stressed with different concentrations of fungal
toxin filtrate. Blast-resistant lines of rice were developed by in vitro selection with toxin filtrate and afterward,
their callus induction and their regeneration were tested. The percentage of regenerating calli greatly varied
depending on variety and toxin concentration. The reduction of regeneration percentages induced by toxin
filtrate was more pronounced in the more sensitive variety, Riho. Protein electrophoresis of regenerated plants
which were tested for blast resistance in the R2 generation in the greenhouse, revealed six additional protein
bands as compared to their original susceptible cultivars. Esterase patterns showed new enzyme bands in the
resistant R2 plants, Alcohol dehydrogenase patterns displayed two distinct bands at two activity zones; these
patterns possibly occurred due to a genetic activation mechanism which affected blast gene(s) expression. The
blast resistant lines should be tested under field conditions before using them in rice blast breeding programs.
Key words: Fungal toxin filtrate, in vitro selection, Oryza sativa, Pyricularia oryza, somaclonal variation, ,
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Abstract
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Cite this Reference:
Antar N. El-Banna and Ismael A. Khatab, . Biochemical Characterization of Rice Somaclones Resistant to Blast. Current Research Journal of Biological Sciences, (2): 137-142.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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