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Abstract
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Article Information:
Response of Bread Wheat Genotypes to Immature Embryo Culture, Callus Induction and Drought Stress
Parvin Elyasi, Ezatollah Farshadfar and Mostafat Aghaee
Corresponding Author: Ezatollah Farshadfar
Submitted: November 28, 2011
Accepted: December 27, 2011
Published: July 10, 2012 |
Abstract:
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In order to evaluate the response of twenty genotypes of bread wheat (Triticum aestivum L.) to callus
induction and in vitro drought stress. The immature embryos of wheat were used in a Completely Randomized
Design (CRD) with six replications for callus induction and a 20×2 factorial experiment based on CRD design
with three replications was carried out for response of genotypes to in vitro drought stress at the Agricultural
College of Razi University, Kermanshah, Iran during 2010-2011. Significant differences were observed among
the genotypes for Callus Growth Rate (CGR), Relative Fresh Weight Growth (RFWG), Relative Growth Rate
(RGR) and Percentage of Callus Induction (PCI) indicating the presence of genetic variability, different
responses of bread wheat genotypes to callus induction and possible selection of callus induction at in vitro
level using immature embryos. Mean comparison of the traits measured in callus induction showed that
genotypes 1 and 6 had the highest PCI (100%). Analysis of variance for CGR, RFWG and RGR, Relative Water
Content (RWC), Percent of Callus Chlorosis (PCC) and Proline Content (PC) exhibited significant differences
among the genotypes for all the characters in the stress condition (15% PEG). Screening drought tolerant
genotypes and in vitro indicators of drought tolerance using mean rank, standard deviation of ranks and biplot
analysis, discriminated genotypes (6), (19) and (1) as the most drought tolerant.
Key words: Biplot analysis, embryo culture, in vitro indicators of drought tolerance, screening techniques, , ,
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Cite this Reference:
Parvin Elyasi, Ezatollah Farshadfar and Mostafat Aghaee, . Response of Bread Wheat Genotypes to Immature Embryo Culture, Callus Induction and Drought Stress. Current Research Journal of Biological Sciences, (4): 372-380.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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