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Abstract
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Article Information:
Isolation and Screening of Keratinolytic Actinobacteria form Keratin Waste Dumped Soil in Tiruchirappalli and Nammakkal, Tamil Nadu, India
Subhasish Saha and Dharumadurai Dhanasekaran
Corresponding Author: Dhanasekaran Dharumadurai
Submitted: 2009 November, 14
Accepted: 2009 December, 07
Published: 2010 March, 10 |
Abstract:
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The aim of this study was to isolate keratinolytic Actinobacteria from feather dumping soil. Feather
dumping soil was collected from several areas in T iruchirappalli, Nammakkal, Tamil Nadu, and India. Twenty
two isolates were selected after growth on Bennett’s agar and they named as SD1 to SD22. All the twenty two
isolates were subjected for primary screening on milk agar plates and among twenty two isolates ten were
showing proteolytic activity in terms of making clear zone surrounding their colony on the Milk agar medium.
The ten positive isolates were again subjected for the secondary screening on Feather Broth and three isolates,
SD5, SD6 and SD7 were showing degradation of feather during their growth. Though the degradation process
was taking long time, all these three isolates achieved complete degradation of feather between 20 to 25 days
and they used feather as the sole organic source for carbon, sulfur and energy. These novel keratinolytic
Actinobacterial isolates have potential biotechnological use in processes involving keratin hydrolysis.
Key words: Bennett’s Agar, keratin hydrolysis, keratinolytic Actinobacteria, milk agar, modified basal liquid medium, ,
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Cite this Reference:
Subhasish Saha and Dharumadurai Dhanasekaran, . Isolation and Screening of Keratinolytic Actinobacteria form Keratin Waste Dumped Soil in Tiruchirappalli and Nammakkal, Tamil Nadu, India. Current Research Journal of Biological Sciences, (2): Page No: 124-131.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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