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Abstract
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Article Information:
Optimization of Protocol to Enhance the Micro Propagation of Lavender Species
N. Jahan, R. Mustafa, M.A. Zaidi, A. Mansoor, J. Khan and D. Baluch
Corresponding Author: N. Jahan
Submitted: November 18, 2011
Accepted: December 20, 2011
Published: April 05, 2012 |
Abstract:
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Tissue culture is a fascinating tool which allows the rapid production of genetically identical, high
yield and disease resistance, long shelf life plants using relatively small amounts of water, space, supplies and
time. The present research work involves, shoot induction, multiple shoot formation and callus induction of
English and French lavender. In our studies shoot induction response from different explants showed that
Murashige Skoog (MS) medium (MS+1 mg/L) IBA, NAA, Kinetin proved best for shoot initiation. Maximum
numbers of multiple shoots (12) were obtained in this media at 7th sub culturing. Callus induction response
showed that the MS medium supplemented with combination of hormones Naphthalene acetic acid (NAA+ 2,
4-D) prove to be best for callusing in lavender plant. Temperature variations also affected the growth and callus
induction from inoculated explants best temperature was 23ºC.
Key words: Callus, explants, kinetin, lavender, Murashige Skoog (MS), Naphthalene Acetic Acid (NAA), tissue culture
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Cite this Reference:
N. Jahan, R. Mustafa, M.A. Zaidi, A. Mansoor, J. Khan and D. Baluch, . Optimization of Protocol to Enhance the Micro Propagation of Lavender Species. Current Research Journal of Biological Sciences, (3): 258-260.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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