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Abstract
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Article Information:
Molecular Study of nifH1, nifH2, nifH3, nifU, nifV, VF Genes and Classical Approach Cared out to Identification of Azotobacter chrococcum from Soil
Adel Kamal Khider
Corresponding Author: Adel Kamal Khider
Submitted: May 24, 2012
Accepted: June 23, 2012
Published: September 20, 2012 |
Abstract:
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The present study aimed to compare classical approach with molecular based method for identification of
Azotobacter chrococcum from soil samples. A. chrococcum was isolated from soil source in Erbil city, Iraq. They
were cultivated under laboratory conditions using Nitrogen free Azotobacter specific medium. A. chrococcum was
present in all soil samples. result shows that A. chrococcum were rod shape, motility occur through the use of
peritrichous flagella, cysts-forming, positive to oxidase, catalase and tryptophanase test, unable to liquefy gelatin,
with insoluble brown or brown-black pigmentation and darken with age. Utilized starch, sucrose, mannitol and
moloanat, but not rhamnose. molecular method based on detection of nifgenes have been successfully applied to
describe A. chrococcum isolated from soil. The PCR products for nifH1 1102bp, nifH2 246bp, nifH3 128bp, nifU
930bp, nifV 1146bp and VF gene 594bp were detected on gel electrophoresis, while no bands observed for negative
control. The isolated bacteria considered Azotobacte chrococcum belonging to Genus Azotobacter.
Key words: Azotobacter, Azotobacter chrococcum, nif gene, nitrogen fixation, PCR, ,
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Cite this Reference:
Adel Kamal Khider, . Molecular Study of nifH1, nifH2, nifH3, nifU, nifV, VF Genes and Classical Approach Cared out to Identification of Azotobacter chrococcum from Soil. Current Research Journal of Biological Sciences, (5): 570-577.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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