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Abstract
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Article Information:
Biochemical Characterization of Protein Isolated from Seaweed, Gracilaria edulis
S. Boobathy, P. Soundarapandian, M. Prithivraj and V. Gunasundari
Corresponding Author: P. Soundarapandian
Submitted: 2009 August, 26
Accepted: 2009 September, 24
Published: 2010 January, 05 |
Abstract:
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The Seaweed, Gracilaria edulis, collected from Mandapam coast, was studied for Biochemical
characterization of protein. Aqueous and methanol extracts yielded a total amount of 6.3 and 5.7 from 500 g
of seaweed respectively. Crude protein extracts from the seaweed was 1.9 mg/mL in aqueous and 2.7 mg/mL
in methanol extract respectively. The partial purification of protein is done by using DEAE cellulose. The
aqueous and methanol extracts showed highest antioxidant activity in DPPH (95 and 82%). On SDS-PAGE the
crude protein yielded four well defined bands at 31.4, 69.5, 92.7 kDa in both the extracts. The Fatty acid profile
showed that myristic acid was dominant in chloroform extract and palmitic acid was dominant in aqueous
extract.
Key words: Characterization, antioxidant, fatty acid, Gracilaria edulis, palmitic acid, ,
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Abstract
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Cite this Reference:
S. Boobathy, P. Soundarapandian, M. Prithivraj and V. Gunasundari, . Biochemical Characterization of Protein Isolated from Seaweed, Gracilaria edulis. Current Research Journal of Biological Sciences, (1): Page No: 35-37.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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