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Article Information:
Effect of Various Thawing Times and Temperatures on Frozen Semen Quality of Friesian Bulls in Iraq
Kreem Iwaid Al-Badry
Corresponding Author: Kreem Iwaid Al-Badry
Submitted: September 22, 2012
Accepted: November 02, 2012
Published: December 20, 2012 |
Abstract:
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This experiment was designed to determine the best practical method of semen thawing which could be applied to frozen semen in straw obtained from Holstein-Friesian produced by Artificial Insemination Center-Iraq. frozen Semen was thawed in the following procedures: 5°C for 30 min, 37°C for 20 sec, 37°C for 30 sec and 60°C for 8 sec and motility, live, morphology and Post-thaw livability of sperm cells was assessed by determining the percentage of progressively motile sperm at 0, 2 and 4 h of incubation at 37°C. Results revealed that motility, live, abnormality, intact acrosoma and Post-thaw livability of sperm cells, were significantly (p<0.05) higher for the 37°C for 30 sec and 60°C for 8 sec than the other thawing methods and post-thaw semen can maintained at water bath 37°C until 3 h. Also thawing procedures at 37°C for 30 sec it is recommended to use in Iraq because it was showing good quality of post thawing semen and easy to used in field. In addition to result indicated that thawing at 60°C for 8 sec lead to best results but difficult practically used in field.
Key words: Friesian bull, frozen semen, temperature, thawing time , , ,
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Abstract
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Cite this Reference:
Kreem Iwaid Al-Badry, . Effect of Various Thawing Times and Temperatures on Frozen Semen Quality of Friesian Bulls in Iraq. International Journal of Animal and Veterinary Advances, (6): 384-388.
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ISSN (Online): 2041-2908
ISSN (Print): 2041-2894 |
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