Abstract
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Article Information:
Inulinase Production by a Mexican Semi-Desert Xerophylic Penicillium citrinum Strain under Submerged Culture
Adriana C. Flores-Gallegos, Jesús Morlett-Chávez, Cristóbal N. Aguilar and Raúl Rodríguez-Herrera
Corresponding Author: Adriana C. Flores-Gallegos
Submitted: 2011 December, 20
Accepted: 2011 January, 21
Published: 2012 February, 15 |
Abstract:
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The aim of the study was to produce inulinase under submerged culture (SmC) by a xerophylic
fungal strain isolated from the Mexican semi-dessert and to verify its potential as an industrial inulinase
producer. This enzyme can be obtained from microorganisms that live in close association with inulin plant
store tissues. Inulin is a widespread plant polyfructan that serves as a storage polysaccharide in several plants
and its depolymerization involves the action of inulinase. Inulinases are classified among hydrolases and target
on the β-(2,1)-linkage of inulin which is hydrolyzed into fructose and glucose. These carbohydrates are
promising candidates to be used as food ingredients and in production of fermenting sugars. However, their
utilization is limited due to the high cost of their production thus it is necessary to find new inulinase producer
strains with high productivity and desirable characteristics for industrial production. In the present study,
Penicillium citrinum ESS produced extracellular inulinase with the highest enzymatic activity (18.7 U/L) at
48 h, 30°C and 25 rpm, respectively. Moreover, the medium that we propose for inulinase production has a
simpler chemical composition than other reported in previous works. Even further improvements on the process
are needed, within the experimental limits of this study, it was possible to demonstrate that the xerophylic
fungal strain Penicillium citrinum ESS isolated from Mexican semi-dessert could compete favorably with other
fungal strains reported in literature to produce inulinase industrially.
Key words: Inulin, inulinase, Mexican semi-dessert, Penicillium citrinum ESS, submerged culture, xerophylic,
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Cite this Reference:
Adriana C. Flores-Gallegos, Jesús Morlett-Chávez, Cristóbal N. Aguilar and Raúl Rodríguez-Herrera, . Inulinase Production by a Mexican Semi-Desert Xerophylic Penicillium citrinum Strain under Submerged Culture. Advance Journal of Food Science and Technology, (1): 46-50.
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ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
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