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Abstract
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Article Information:
Azurin as Antitumor Protein and its Effect on the Cancer Cell Lines
Mervat S. Mohamed, Said A. Fattah and Howayada M. Mostafa
Corresponding Author: Mervat S. Mohamed
Submitted: 2010 September, 10
Accepted: 2010 October, 09
Published: 2010 November, 25 |
Abstract:
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As a low molecular weight redox protein was elaborated from the pathogenic bacteria Pseudomonas
aeruginosa, azurin is one of the representative bacterial products used in the treatment of tumours. In this study,
Pseudomonas aeruginosa was isolated from lungs of cystic fibrosis patients in Egypt, and identified by 16S
rRNA. Azurin gene was amplified using the suitable PCR programme. The gene was cloned into pGEM-T easy
vector and sequenced. Then subcloned into the overexpression vector pET-28a (+) using E. coli BL21 as
expression bacteria. The SDS-PAGE analysis showed that the gene overexpressed in E. coli. The recombinant
protein was purified by one step purification using Ni++ resin. The effect of azurin on proliferation and apoptosis
of human breast cancer cell line (MCF7), human hepatocellular carcinoma cell line (HEPG2) and human colon
carcinoma cell line (HCT116) and human normal melanocytes (HFB4) cell line was studied. The results showed
that azurin strongly inhibited the proliferation of both colon carcinoma cell line and breast cancer cell line.
Whereas unclear result of the effect of azurin on hepatocellular carcinoma cell line was exhibited. At the same
time, azurin didn’t show any effect on the human normal melanocytes.
Key words: Antitumor, azurin, cell lines, Pseudomonas aeruginosa, , ,
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Abstract
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Cite this Reference:
Mervat S. Mohamed, Said A. Fattah and Howayada M. Mostafa, . Azurin as Antitumor Protein and its Effect on the Cancer Cell Lines. Current Research Journal of Biological Sciences, (6): 396-401.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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