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Abstract
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Article Information:
Amylase from an Estuarine Bacillus megaterium
N. Sajitha, V. Vasanthabharathi, R. Lakshminarayanan and S. Jayalakshmi
Corresponding Author: N. Sajitha
Submitted: 2010 October, 19
Accepted: 2010 November, 18
Published: 2011 March, 05 |
Abstract:
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The main aim of this study is to isolate and identify the amylase producing bacteria from the
estuarine environment. The water and sediment samples were collected from the uppanar estuary and the
potential strains of amylase production were screened in starch agar plates. Totally 12 strains were collected,
among them Bacillus megaterium exhibited the larges zone. Hence these potential strains were used for further
optimization, production and purification of amylase enzyme. Optimizations and enzyme estimation were done
through various factors. Here the enzyme productions were maximum at 35ºC, pH 7.5 and 30 ppt salt
concentration.
Key words: Isolation , optimization, purification, screening, , ,
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Abstract
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Cite this Reference:
N. Sajitha, V. Vasanthabharathi, R. Lakshminarayanan and S. Jayalakshmi, . Amylase from an Estuarine Bacillus megaterium. Current Research Journal of Biological Sciences, (2): 110-115.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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