Abstract
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Article Information:
Genetic Analysis of Some Commonly Grown Genotypes of Maize in Pakistan Using Maize Specific Simple Sequence Repeat (SSR) Primers
Sadaf Bibi, Habib Ahmad, Sajid Ghafoor, Khaist Begum, Sahib Gul Afridi and Imtiaz Ahmed Khan
Corresponding Author: Imtiaz Ahmed Khan
Submitted: 2010 June, 04
Accepted: 2010 July, 03
Published: 2010 July, 20 |
Abstract:
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Maize (Zea mays) belongs to the family gramineae (poaceae), genus Zea and species mays. The
genome of maize comprises approximately 2500 million base pairs. During the present study, maize specific
Simple Sequence Repeat (SSR) primers were used to estimate genetic distances among fourteen
varieties/genotypes of maize commonly cultivated in Pakistan. On an average, 3.2 alleles per genotype were
amplified. Average genetic distance estimated using the four SSR primer sets ranged from 0 - 53%.
Dendogram based on DNA data showed that the fourteen genotypes were clustered in six groups A, B, C, D,
E and F comprising 3, 4, 2, 2, 2 and 1 genotypes respectively. It has been concluded that genotypes 6098 and
Ev- 1097 were most distantly related to each other.
Key words: Dendrogram, genetic diversity, PCR, phylogenetic elaboration, simple sequence repeats, Zea mays,
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Cite this Reference:
Sadaf Bibi, Habib Ahmad, Sajid Ghafoor, Khaist Begum, Sahib Gul Afridi and Imtiaz Ahmed Khan, . Genetic Analysis of Some Commonly Grown Genotypes of Maize in Pakistan Using Maize Specific Simple Sequence Repeat (SSR) Primers. Current Research Journal of Biological Sciences, (4): Page No: 259-261.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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