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Abstract
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Article Information:
Sequence Assigning of the Growth Differentiation Factor-9 (GDF9) Gene in Markhoz Goats
Sadegh Fathi Yosefabad, Jalal Shayegh, Naser Maheri-Sis, Abolfazl Barzegari, Abolfazl Gorbani, Abdulreza Biroonara, Hourad Ghoreishi and Alireza Lotfi
Corresponding Author: Sadegh Fathi Yosefabad
Submitted: 2011 May, 17
Accepted: 2011 June, 02
Published: 2011 August, 30 |
Abstract:
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This study was conducted to sequence the Growth Differentiation Factor-9 (GDF9) from
Transforming Growth Factor beta (TGF β) super family in Markhoz breed of goat. This gene has vital role in
ovarian follicle development, ovulation rate and fertility. Blood samples were collected from Sanandaj Markhoz
goat breeding station and DNA was extracted using standard phenol chloroform method. The primers for exon
1 and 2 were designed and used for amplification of the gene fragments through the simple PCR procedure. The
length of exon 1 and 2 are 463bp and 997bp, respectively. Sequence detection was performed after
amplification of the gene segments. A mutation at exon 1 in base No.233 with C¦A type and two mutations at
exon 2, one in base No.354 with T¦C type and another in base No.594 with A¦C type were recognized. These
mutations were documented and recorded in the NCBI gene bank with number of GU784823.
Key words: GDF9, markhoz goats, ovulation rate, PCR, sequencing, TGFβ,
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Cite this Reference:
Sadegh Fathi Yosefabad, Jalal Shayegh, Naser Maheri-Sis, Abolfazl Barzegari, Abolfazl Gorbani, Abdulreza Biroonara, Hourad Ghoreishi and Alireza Lotfi, . Sequence Assigning of the Growth Differentiation Factor-9 (GDF9) Gene in Markhoz Goats. International Journal of Animal and Veterinary Advances, (4): 274-276.
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ISSN (Online): 2041-2908
ISSN (Print): 2041-2894 |
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