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Abstract
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Article Information:
Studies on the Interactions between Pto and Pti1s Proteins from Maize by a Yeast Two-Hybrid System
Yong Zhu, Xiao-yan Sun, Beng Wang, Zhong-jian Song, Hua-wen Zou
Corresponding Author: Hua-wen Zou
Submitted: September 11, 2012
Accepted: October 19, 2012
Published: December 20, 2012 |
Abstract:
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To check whether the protein ZmPto can interact with the protein ZmPti1s, in this study, the gene
sequences of ZmPto and ZmPti1a, ZmPti1b were inserted into yeast expression vectors pEG202 and pJG4-5,
respectively. Yeast two-hybrid was then conducted to verify their interactions. Results showed that yeast strains
neither carrying empty plasmid nor carrying only one single fusion sequence of ZmPti1 or ZmPto can activate
reporter genes. Only the yeast strains carrying both fusion sequence of ZmPti1 and ZmPto can activate reporter
genes indicating that ZmPto can interact directly with ZmPti1 in the yeast two-hybrid system.
Key words: Maize, Yeast two-hybrid system, ZmPto, ZmPti1, , , ,
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Cite this Reference:
Yong Zhu, Xiao-yan Sun, Beng Wang, Zhong-jian Song, Hua-wen Zou, . Studies on the Interactions between Pto and Pti1s Proteins from Maize by a Yeast Two-Hybrid System. Advance Journal of Food Science and Technology, (6): 389-392.
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ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
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