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Abstract
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Article Information:
Characterization of Amylase, Cellulase and Proteinase Enzyme in Stomach and Intestine of the Mekong Giant Catfish Fed with Various Diets Consisting of Spirulina
Sudaporn Tongsiri, Kringsak Mang-Amphan and Yuwadee Peerapornpisal
Corresponding Author: Sudaporn Tongsiri
Submitted: 2010 May, 29
Accepted: 2010 June, 15
Published: 2010 July, 20 |
Abstract:
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The amylase, cellulase and proteinase enzyme from the stomach and intestine of the Mekong Giant
Catfish that had been fed with various diets consisting of Spirulina, were studied at pH 2-12 and at temperatures
between 25-80ºC. This study found that; amylase activities of the stomach were alkaline amylase and the
optimal temperatures to be 25 and 50ºC. Amylase activities of the intestine were neutral amylase as well as
alkaline amylase and the optimal temperature range was at 25-30ºC. The cellulase activities were acidic
cellulase and alkaline cellulase in both the stomach and the intestine. In the stomach, the acidic cellulase
showed the optimal temperatures to be at 30, 40 and 50ºC, however alkaline cellulase were 30 and 50ºC. In the
intestine, acidic cellulase showed the optimal temperatures to be at 25, 60 and 70ºC and the alkaline cellulase
to be 80ºC. For proteinase activity, the stomach was acidic proteinase and alkaline proteinase, but the intestine
was alkaline proteinase. The acidic proteinase activities of the stomach showed the optimal temperatures to be
at 40, 50 and 60ºC and the alkaline proteinase activity proved to be at 40, 60 and 70ºC. The optimal conditions
for amylase enzymes showed the higher specific activity in the intestine than in the stomach including the
proteinase enzyme. At room temperature (25-30ºC), amylase and proteinase specific acitivity dominated in the
intestine, while cellulase specific activity dominated in the stomach.
Key words: Amylase, cellulase, mekong giant catfish, proteinase, , ,
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Abstract
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Cite this Reference:
Sudaporn Tongsiri, Kringsak Mang-Amphan and Yuwadee Peerapornpisal, . Characterization of Amylase, Cellulase and Proteinase Enzyme in Stomach and Intestine of the Mekong Giant Catfish Fed with Various Diets Consisting of Spirulina. Current Research Journal of Biological Sciences, (4): Page No: 268-274.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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