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Abstract
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Article Information:
Cytotoxicity of Curcumin against Leukemic Cell Lines via Apoptosis Activity
J.H. Farah, S.S. Muayad and H. Aljewari
Corresponding Author: J.H. Farah
Submitted: 2011 November, 09
Accepted: 2011 November, 29
Published: 2012 January, 20 |
Abstract:
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The aimed of this study was to evaluate curcumin as anticancer agent. The apoptogenic and
Cytotoxic activity of curcumin were investigated against two human leukemic cell lines-U937 and Molt4.
Cytotoxic activity was performed by using [3-[4, 5-dimethylthiazol-2yl] 2, 5 diphenyltetrazolium bromide]
MTT assay. Also 4, 6-diamidino-2-phenylindole di-hydrochloride [DAPI] staining was used as a DNA-binding
fluorochome for in vitro apoptosis detection. Our result showed that curcumin have cytotoxic activity to both
U937, Molt4 cell lines with 9.25 and 6.59/mL IC50 for U937 and Molt4 respectively. Otherwise curcumin
was found to possess apoptotic activities which were 80.02 and 55.06% for U937 and Molt4, respectively
compare with 90 and 88% against U937 and Molt4 in melphalan and the process of apoptosis was
characterized by several morphological changes such as nuclear fragmentation. In summary, curcumin
possessed anticancer activity by inhibition cell growth and metabolic activity of the leukemic cells and showed
characteristic features of apoptosis. Further biological testing on the efficacy of purified curcumin will be
conducted to get better understand of the mechanism of anticancer activity.
Key words: Apoptosis, curcumin, cytotoxicity, DAPI stain, IC50, leukemic cells, MTT assay
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Abstract
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Cite this Reference:
J.H. Farah, S.S. Muayad and H. Aljewari, . Cytotoxicity of Curcumin against Leukemic Cell Lines via Apoptosis Activity. Current Research Journal of Biological Sciences, (1): 60-64.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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