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Abstract
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Article Information:
Phylogenetic Analysis of Enterococcus, Lactobacillus and Streptococcus Strains on the Basis of abc (Atp Binding Protein) Gene Sequences
R. Dewangan, A. Patel, S. Khatri, J. Choubey, M.K. Verma, S. Kumar Gupta and V. Rishi
Corresponding Author: Ashish Patel
Submitted: 2009 July, 07
Accepted: 2009 July, 27
Published: |
Abstract:
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Phylogenetic analysis of about 14 strains of Enterococcus, Lactobacillus and Streptococcus was
carried out using the nucleotide sequence of the gene for abc (ATP binding protein), The result establish a new
phylogenetic tree for the classification of Enterococcus, Lactobacillus and Streptococcus. In comparison with
16s rRNA analysis, the abc sequence indicated a greater evolutionary divergence for the bacteria. Thus, in
screening for the presence of bacteria, the abc gene might be a useful tool for differentiating between closely
related species of bacteria such as Lactobacillus species. and Streptococcus. At present, 16s rRNA sequence
analysis is an accurate and rapid method for identifyieng most unknown bacteria to the genus level because
the highly conserved 16Ss rRNA region is easy to amplify; however, analysis of the more variable abc
sequence region can identify unknown bacteria to the species level. In summary, we have shown that abc
sequence analysis is a useful alternative to 16s rRNA analysis for constructing the phylogenetic relationships
of bacteria, in particular for the classification of closely related bacterial species.
Key words: Phylogeny, Enterococcus, binding protein, Lactobacillus, Streptococcus, ,
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Cite this Reference:
R. Dewangan, A. Patel, S. Khatri, J. Choubey, M.K. Verma, S. Kumar Gupta and V. Rishi, . Phylogenetic Analysis of Enterococcus, Lactobacillus and Streptococcus Strains on the Basis of abc (Atp Binding Protein) Gene Sequences. Current Research Journal of Biological Sciences, (3): Page No: 127-130.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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