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Abstract
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Article Information:
Caffeine Alters Skeletal Muscle Contraction by Opening of Calcium Ion Channels
Kolawole Victor Olorunshola and L.N. Achie
Corresponding Author: Dr. Kolawole Victor Olorunshola
Submitted: 2011 June, 07
Accepted: 2011 September, 07
Published: 2011 September, 10 |
Abstract:
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The aim of this study was to investigate the effect of caffeine on the amplitude and rate of skeletal
muscle contraction using frog sciatic nerve-gastrocnemius muscle model. Caffeine is a xanthine alkaloid whose
use is widely unregulated. It is taken as a central nervous system stimulant in various foods and drinks. The
effect of caffeine on skeletal muscle contraction and a possible elucidation of its mechanism of action were
investigated. The sciatic nerve-gastrocnemius muscle preparation of the frog mounted on a kymograph was
utilized. Varying doses of caffeine was added to the organ bath at 5, 10, 15, 20 and 25 mg/mL and its effect on
skeletal muscle contraction was studied. The effects of caffeine preceded by administration of acetylcholine,
atropine, nifedipine, magnesium chloride and calcium gluconate at 25 mg/mL were also studied. A dose
dependent increase in skeletal muscle contraction (25.25±0.48, 49.00±1.23, 52.38±2.58, 59.25±1.11 and
68.50±0.87 mV; p<0.05) was observed on administration of increasing doses (5, 10, 15, 20 and 25 mg/mL,
respectively) of caffeine respectively. While a significant reduction (0.90±0.04 mV) and increase (77.50±1.56 mV) in strength of contraction was observed on administration of nifedipine and calcium gluconate
respectively. Administration of magnesium chloride caused a significant decrease in the strength of contraction
(28.25±5.01) as compared to control. However, there was no significant difference in the contraction period
and relaxation period between the treatment groups. The findings imply that caffeine increases skeletal muscle
contraction and suggests it exerts the effect through increasing calcium ion release.
Key words: Caffeine, calcium ion, magnesium chloride, nifedipine, skeletal muscle, ,
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Cite this Reference:
Kolawole Victor Olorunshola and L.N. Achie, . Caffeine Alters Skeletal Muscle Contraction by Opening of Calcium Ion Channels. Current Research Journal of Biological Sciences, (5): 521-525.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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