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     Current Research Journal of Biological Sciences

    Abstract
2012(Vol.4, Issue:4)
Article Information:

Reconsideration in Using Citric Acid as Vase Solution Preservative for Cut Rose Flowers

Mohammad Mahdi Jowkar, Mohsen Kafi, Ahmad Khalighi and Nader Hasanzadeh
Corresponding Author:  Mohammad Mahdi Jowkar 
Submitted: February 16, 2012
Accepted: March 24, 2012
Published: July 10, 2012
Abstract:
The major cause of vase life reduction in cut flowers is water relation interruption which is mostly due to vase solution microbial proliferation and consequently vascular occlusion resulting in solution uptake reduction. In order to reduce vase solution microbial count, most preservatives incorporate acidifying agents mostly citric acid. Beside microbial proliferation control, biocides could affect cut flower’s quality and physiology in various aspects. In order to study the biocidal effect of citric acid, ‘Cherry Brandy’ roses were treated in a completely randomized design with: citric acid (300, 600 and 900 mg/L, respectively) or sterilized distilled water (control). Effects of citric acid application as vase solution biocide and its impact on vase life, water relation, vase solution microbial kind and population beside different physiological parameters such as chlorophyll degradation, chlorophyll fluorescence and membrane permeability were investigated during this study. Results indicated that citric acid increased vase life (although insignificantly) and amazingly its’ application resulted in better fresh appearance during last days of vase life. Although microbial control was not desirable, but it was achieved to some extent by citric acid application. Citric acid resulted in higher fresh weight increment. But fresh weight was lost more rapidly compared to control. This was while citric acid treated flowers had lower solution up take at most stages of vase life. Five different micro-organisms were observed in citric acid vase solutions which were: 2 strains of Fusarium solani and three isolates of Bacillus, Coccus and Streptomyces sp. Ion leakage trend showed a steady increase during vase life and was significantly increased by citric acid application during the last days of vase life as membrane permeability and vase life decrease. Citric acid significantly increased leaf chlorophyll content of treated flowers while it resulted in chlorophyll fluorescence reduction during vase life.

Key words:  Bacillus, chlorophyll content, chlorophyll fluorescence, Fusarium solani, ion leakage, microbial count, microbial kind
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Cite this Reference:
Mohammad Mahdi Jowkar, Mohsen Kafi, Ahmad Khalighi and Nader Hasanzadeh, . Reconsideration in Using Citric Acid as Vase Solution Preservative for Cut Rose Flowers. Current Research Journal of Biological Sciences, (4): 427-436.
ISSN (Online):  2041-0778
ISSN (Print):   2041-076X
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