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Abstract
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Article Information:
Chromosomal nif Genes Transfer by Conjugation in Nitrogen Fixing Azotobacter chroococcum to Lactobacillus plantarium
Adel Kamal Khider and Aras Muhammad Khidher
Corresponding Author: Adel Kamal Khider
Submitted: 2011 January, 08
Accepted: 2011 February, 07
Published: 2011 March, 05 |
Abstract:
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To determine the possibility of transferring chromosomal nitrogen fixation genes (nif genes) from
Azotobacter chroococcum to Lactobacillus planetarium, a total of 72 Azotobacter chroococcum isolated from
Erbil governorate, Iraq were culturally, morphologically and biochemically characterized. Genes for
atmospheric nitrogen fixation, located on the chromosome of Azotobacter chroococcum isolates were
transferred by conjugation process to a recipient Lactobacillus plantarium isolated from Erbil city soils. The
chromosomal genes transferred were verified by analysis of the genomes of donor, recipient and putative
transconjugants, by polymorphism of DNA bands obtained through amplification of nifH1, nifH2, nifH3, nifU
and nifV genes by PCR technique. The transconjugant cells promote an efficient fixation of nitrogen in liquid
cultures fixed 0.2% nitrogen, and in the soil as inoculums of wheat plants, fixed 0.31% nitrogen and solublized
11.71 ppm phosphorus, beside all advantages of Lactic acid bacteria, and probably to be used as inoculums for
both nitrogen fixation and solublizing insoluble phosphorus components, and used as biofertilizers
Key words: Azotobacter chroococcum, conjugation and gene transfer, Lactobacillus plantarium, nif genes, nitrogen fixation, ,
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Cite this Reference:
Adel Kamal Khider and Aras Muhammad Khidher, . Chromosomal nif Genes Transfer by Conjugation in Nitrogen Fixing Azotobacter chroococcum to Lactobacillus plantarium. Current Research Journal of Biological Sciences, (2): 155-164.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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