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Abstract
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Article Information:
Rapid Detection of the Philippine Isolate of Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) in Shrimp, Penaeus monodon Using Loop-Mediated Isothermal Amplification (LAMP)
Christopher Marlowe A. Caipang, May Flor J. Sibonga and Jane S. Geduspan
Corresponding Author: Christopher Marlowe A. Caipang
Submitted: May 06, 2012
Accepted: June 01, 2012
Published: June 15, 2012 |
Abstract:
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The aim of this study was to standardize a Loop-Mediated isothermal Amplification (LAMP) assay for
the detection of the Philippine isolate of Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) in
postlarvae of shrimp, Penaeus monodon. The assay was optimized at an incubation time of 1 h at 63oC. The assay
was highly specific for IHHNV and did not cross-react with other shrimp viruses including Hepatopancreatic
Parvovirus (HPV), Monodon Baculovirus (MBV) and White Spot Syndrome Virus (WSSV). The limit of detection
of the IHHNV using the LAMP assay was 10 pg of DNA/mL or 10 fg of the genomic DNA per LAMP reaction and
was 10 times more sensitive than conventional PCR in detecting the viral pathogen from infected samples. These
results demonstrated that LAMP is a simple and sensitive diagnostic technique that has potential application for
routine detection of IHHNV infections in shrimp hatcheries in the Philippines.
Key words: IHHNV, LAMP, Penaeus monodon, shrimp, LAMP, ,
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Abstract
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Cite this Reference:
Christopher Marlowe A. Caipang, May Flor J. Sibonga and Jane S. Geduspan, . Rapid Detection of the Philippine Isolate of Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) in Shrimp, Penaeus monodon Using Loop-Mediated Isothermal Amplification (LAMP). International Journal of Animal and Veterinary Advances, (3): 229-234.
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ISSN (Online): 2041-2908
ISSN (Print): 2041-2894 |
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