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Abstract
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Article Information:
Cloning of Lactate dehydrogenase Gene and Effect on the Waterlogging Tolerance of Brassica napus L.
Ben-bo Xu, Ling-li Xie, Yong Cheng, Guang-yuan Lu, Pu-ying Zheng and Xue-kun Zhang
Corresponding Author: Ben-bo Xu
Submitted: August 31, 2012
Accepted: October 03, 2012
Published: October 20, 2012 |
Abstract:
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To investigate the mechanism on waterlogging tolerance in Brassica napus, 12 B. napus cultivars with
different waterlogging tolerance were used in the research and Waterlogging Tolerance Index (WTI) was calculated
by multiplying relative percentage germination and the relative seedling height. The results indicated that Lactate
Dehydrogenase (LDH) enzyme activity rapidly increased at 24 h after waterlogging treatment and reached peak
between 48-72 h. WTI was correlated with LDH enzyme activity at 24 h after water logging treatment and the
correlation coefficient between them was 0.84. Transcription level of the BnLDH had significant difference in the
12 lines after waterlogging treatment. BnLDH expression level was very low before waterlogging treatment and
induced by waterlogging treatment and arrived at peak at 48 h. Correlation analysis indicated correlation coefficient
between WTI and BnLDH expression at 24 and 48 h after waterlogging treatment was 0.56 and 0.72, respectively.
An LDH gene, denoted BnLDH-1, was cloned from oilseeds by the Rapid Amplification of CDNA Ends (RACE)
from 12 materials and the results indicated all of them had same protein sequence.
Key words: Brassica napus L., cloning, lactate dehydrogenase, waterlogging tolerance, waterlogging tolerance, index lipase,
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Cite this Reference:
Ben-bo Xu, Ling-li Xie, Yong Cheng, Guang-yuan Lu, Pu-ying Zheng and Xue-kun Zhang, . Cloning of Lactate dehydrogenase Gene and Effect on the Waterlogging Tolerance of Brassica napus L.. Advance Journal of Food Science and Technology, (5): 332-336.
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ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
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