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Abstract
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Article Information:
The Usage of ß-glucan Extracted from Local Mushroom as Immunomodulator
Mouruj A. Alaubydi and Reema M. Abed
Corresponding Author: Mouruj A. Alaubydi
Submitted: 2011 May, 21
Accepted: 2011 August, 05
Published: 2011 September, 10 |
Abstract:
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The present study was conducted to investigate the active compound of local cultured mushroom
(β-glucan), and studied it effect immunologically on some organs (liver, spleen, small intestine). The results
of drying 1 kg of fresh mushroom was 80 g dried material, the carbohydrates (polysaccharides) were extracted
from the dried powder of mushroom by ethanol (99%).The carbohydrate and protein concentration were 88,
1.12 mg/mL, respectively, then the purification method was done for the extracted material, which were
separated in to three main peaks, the carbohydrate concentration measured which were 490, 405 and 315
mg/mL, respectively, while Lowry method was detected the protein concentration for these peaks which were
0.0038, 0.0032 and 0.0023 mg/mL, respectively. HPLC carried out to identify β-glucan for these peaks. The
results showed that the first peak was close one to standard β-glucan. The three peaks were examined as
immunomodulator for lab. Animals (mice) that were divided into three groups according to the peaks of ion
exchange diagram plus group of control. The results of immunization method showed crossly enlargement of
liver, splean and small intestine, for all experimental animals groups, and microscopically changes in the tissues
structure for these organs, generally there were infiltration of mononuclear cells in all these organs especially
for the first group.
Key words: β-glucan, exchange, HPLC, immunomodulator, infilteration, ionic, local mushroom
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Cite this Reference:
Mouruj A. Alaubydi and Reema M. Abed, . The Usage of ß-glucan Extracted from Local Mushroom as Immunomodulator. Current Research Journal of Biological Sciences, (5): 535-541.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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